Human membrane-associated ring finger (C3HC4) 8 ELISA Kit | MARCH8 elisa kit

Human E3 ubiquitin-protein ligase MARCH8, MARCH8 ELISA Kit

Cat. Num. AAA9320454

• Gene Names: MARCH8; MIR; c-MIR; RNF178; MARCH-VIII
• Reactivity: Human
• Synonyms: membrane-associated ring finger (C3HC4) 8; Human E3 ubiquitin-protein ligase MARCH8; MARCH8 ELISA Kit; Human E3 ubiquitin-protein ligase MARCH8 (MARCH8) ELISA kit; RP11-67C2.1; MARCH-VIII; MIR; RNF178; c-MIR; c-mir; cellular modulator of immune recognition; cellular modulator of immune recognition (c-MIR) ; membrane-associ; MARCH8 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between this analyte and analogues is observed.

• Samples: Undiluted original Human body fluids, tissue homogenates, secretions or feces samples.
• Assay Type: Sandwich (Quantitative)
• Detection Range: 0.625ng/ml-20ng/ml.
• Sensitivity: 0.1ng/ml.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for MARCH8 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! This kit is intended to be used for determination the level of MARCH8 (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

NCBI and Uniprot Product Information

• NCBI GI #: 50539414
• NCBI GeneID: 220972
• NCBI Accession #: NP_001002266.1
• NCBI GenBank Nucleotide #: NM_001002266.2
• UniProt Accession #: Q5T0T0; Q5T0S8; Q8TC72; B2R8E7; H0Y7C6
• Molecular Weight: 32,965 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase MARCH8 isoform a
• NCBI Official Synonym Full Names: membrane-associated ring finger (C3HC4) 8, E3 ubiquitin protein ligase
• NCBI Official Symbol: MARCH8
• NCBI Official Synonym Symbols: MIR; c-MIR; RNF178; MARCH-VIII
• NCBI Protein Information: E3 ubiquitin-protein ligase MARCH8; RING finger protein 178; membrane-associated RING-CH protein VIII; membrane-associated RING finger protein 8; c-mir, cellular modulator of immune recognition; cellular modulator of immune recognition (c-MIR)
• UniProt Protein Name: E3 ubiquitin-protein ligase MARCH8
• UniProt Gene Name: MARCH8
• UniProt Synonym Gene Names: MIR; RNF178; c-MIR; MARCH-VIII
• UniProt Entry Name: MARH8_HUMAN

NCBI Description

MARCH8 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH8 induces the internalization of several membrane glycoproteins (Goto et al., 2003 [PubMed 12582153]; Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Apr 2010]

Uniprot Description

MARCH8: E3 ubiquitin-protein ligase that mediates ubiquitination of CD86 and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. May also promote ubiquitination and endocytosis of TFRC and FAS.

Protein type: EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase; Ligase; Membrane protein, multi-pass; Membrane protein, integral; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 10q11.21

Cellular Component: cytoplasmic vesicle membrane; early endosome membrane; lysosome; lysosomal membrane; integral to membrane; endosome

Molecular Function: zinc ion binding; ubiquitin-protein ligase activity; ligase activity; MHC class II protein binding

Biological Process: protein polyubiquitination; antigen processing and presentation of peptide antigen via MHC class II; negative regulation of MHC class II biosynthetic process