Human ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast) ELISA Kit | UBE2N elisa kit

Human Ubiquitin-conjugating enzyme E2 N, UBE2N ELISA Kit

Cat. Num. AAA9318911

• Gene Names: UBE2N; UBC13; UbcH13; HEL-S-71; UbcH-ben; UBCHBEN; UBC13
• Reactivity: Human
• Synonyms: ubiquitin-conjugating enzyme E2N (UBC13 homolog; yeast); Human Ubiquitin-conjugating enzyme E2 N; UBE2N ELISA Kit; Human Ubiquitin-conjugating enzyme E2 N (UBE2N) ELISA kit; MGC131857; MGC8489; UBC13; UbcH-ben; bendless-like ubiquitin conjugating enzyme; ubiquitin carrier protein N; ubiquitin-conjugating enzyme E2N; ubiquitin-conjugating enzyme E2N (homologous to yeast UBC; UBE2N elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between this analyte and analogues is observed.

• Samples: Undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
• Assay Type: Sandwich
• Detection Range: 0.625 ng/ml - 20 ng/ml.
• Sensitivity: 0.1 ng/ml.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for UBE2N elisa kit

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! This kit is intended to be used for determination the level of UBE2N (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

NCBI and Uniprot Product Information

• NCBI GI #: 4507793
• NCBI GeneID: 7334
• NCBI Accession #: NP_003339.1
• NCBI GenBank Nucleotide #: NM_003348.3
• UniProt Accession #: P61088; Q16781; Q53Y81
• Molecular Weight: 17,138 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 N
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2N
• NCBI Official Symbol: UBE2N
• NCBI Official Synonym Symbols: UBC13; UbcH13; HEL-S-71; UbcH-ben; UBCHBEN; UBC13
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 N; yeast UBC13 homolog; ubiquitin-protein ligase N; ubiquitin carrier protein N; epididymis secretory protein Li 71; bendless-like ubiquitin conjugating enzyme; bendless-like ubiquitin-conjugating enzyme; ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast); ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13)
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 N
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2N
• UniProt Synonym Gene Names: BLU
• UniProt Entry Name: UBE2N_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair. [provided by RefSeq, Jul 2008]

Uniprot Description

UBE2N: The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Heterodimer with UBE2V2. Interacts (UBE2V2-UBE2N heterodimer) with the E3 ligase STUB1 (via the U-box domain); the complex has a specific 'Lys-63'-linked polyubiquitination activity. Interacts with RNF8 and RNF168. Interacts with RNF11. Interacts with the E3 ligases, HLTF and SHPRH; the interactions promote the 'Lys-63'-linked polyubiquitination of PCNA upon genotoxic stress and lead to DNA repair. Interacts with ARIH2 (via RING-type 2). Belongs to the ubiquitin-conjugating enzyme family.

Protein type: Ubiquitin conjugating system; Ubiquitin ligase; DNA repair, damage; EC 6.3.2.19; Ligase

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: protein complex; cytoplasm; nucleus; cytosol; UBC13-MMS2 complex; ubiquitin ligase complex

Molecular Function: protein binding; ubiquitin binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; regulation of histone ubiquitination; positive regulation of DNA repair; positive regulation of I-kappaB kinase/NF-kappaB cascade; postreplication repair; positive regulation of histone modification; cytokine and chemokine mediated signaling pathway; DNA double-strand break processing; protein ubiquitination; protein modification process; proteolysis; T cell receptor signaling pathway; double-strand break repair via homologous recombination; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 2 signaling pathway; regulation of DNA repair; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; positive regulation of ubiquitin-protein ligase activity; histone ubiquitination; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway