Human Sialyltransferase 4C (SIAT4C) ELISA Kit | SIAT4C elisa kit

Human Sialyltransferase 4C (SIAT4C) ELISA Kit

Cat. Num. AAA9312934

• Gene Names: ST3GAL4; STZ; SAT3; CGS23; SIAT4; NANTA3; SIAT4C; ST3GalIV
• Reactivity: Human
• Synonyms: Sialyltransferase 4C (SIAT4C); Human Sialyltransferase 4C (SIAT4C) ELISA Kit; SIAT4C elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human SIAT4C and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 0.25ng/ml-8ng/ml.
• Sensitivity: 0.1ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated SIAT4C concentrations in Human serum, plasma and other body fluids. Using Purified Human SIAT4C antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SIAT4C and SIAT4C antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SIAT4C in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for SIAT4C elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated SIAT4C concentrations in Human serum, plasma and other body fluids. Using Purified Human SIAT4C antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SIAT4C and SIAT4C antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SIAT4C in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 48145833
• NCBI GeneID: 6484
• UniProt Accession #: Q6IBE6; Q11206
• Molecular Weight: 37,458 Da
• NCBI Official Full Name: SIAT4C
• NCBI Official Synonym Full Names: ST3 beta-galactoside alpha-2,3-sialyltransferase 4
• NCBI Official Symbol: ST3GAL4
• NCBI Official Synonym Symbols: STZ; SAT3; CGS23; SIAT4; NANTA3; SIAT4C; ST3GalIV
• NCBI Protein Information: CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 4; ST-4; SAT-3; SIAT4-C; ST3GalA.2; gal-NAc6S; alpha 2,3-ST 4; alpha 2,3-sialyltransferase IV; alpha-3-N-acetylneuraminyltransferase; beta-galactoside alpha-2,3-sialyltransferase 4; gal-beta-1,4-GalNAc-alpha-2,3-sialyltransferase; sialyltransferase 4C (beta-galactoside alpha-2,3-sialytransferase); sialyltransferase 4C (beta-galactosidase alpha-2,3-sialytransferase)
• UniProt Protein Name: SIAT4C protein
• UniProt Gene Name: SIAT4C
• UniProt Synonym Gene Names: ST3GAL4
• UniProt Entry Name: Q6IBE6_HUMAN

NCBI Description

This gene encodes a member of the glycosyltransferase 29 family, a group of enzymes involved in protein glycosylation. The encoded protein is targeted to Golgi membranes but may be proteolytically processed and secreted. The gene product may also be involved in the increased expression of sialyl Lewis X antigen seen in inflammatory responses. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

ST3GAL4: It may catalyze the formation of the NeuAc-alpha-2,3- Gal-beta-1,3-GalNAc- or NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc- sequences found in terminal carbohydrate groups of glycoproteins and glycolipids. It may be involved in the biosynthesis of the sialyl Lewis X determinant. Also acts on the corresponding 1,3- galactosyl derivative. Belongs to the glycosyltransferase 29 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.4.99.-; Transferase; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Glycan Metabolism - keratan sulfate biosynthesis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q24.2

Cellular Component: Golgi membrane; membrane; integral to Golgi membrane

Molecular Function: monosialoganglioside sialyltransferase activity; beta-galactoside alpha-2,3-sialyltransferase activity

Biological Process: keratan sulfate metabolic process; protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; glycosaminoglycan metabolic process; dolichol-linked oligosaccharide biosynthetic process; carbohydrate metabolic process; keratan sulfate biosynthetic process; protein amino acid N-linked glycosylation via asparagine; pathogenesis; cognition; post-translational protein modification