Human Epstein-Barr virus induced 3 ELISA Kit | EBI3 elisa kit

Human Interleukin-27 subunit beta, EBI3 ELISA Kit

Cat. Num. AAA924266

• Gene Names: EBI3; IL27B; IL-27B
• Reactivity: Human
• Synonyms: Epstein-Barr virus induced 3; Human Interleukin-27 subunit beta; EBI3 ELISA Kit; Human Interleukin-27 subunit beta (EBI3) ELISA kit; IL27B; Epstein-Barr virus induced gene 3; cytokine receptor; interleukin-27 subunit beta; EBI3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human EBI3. No significant cross-reactivity or interference between human EBI3 and analogues was observed.
• Sequence Length: 229

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 15.6 pg/ml -1000 pg/ml.
• Sensitivity: The minimum detectable dose of human EBI3 is typically less than 3.9 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EBI3 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EBI3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EBI3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EBI3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EBI3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 14577917
• NCBI GeneID: 10148
• NCBI Accession #: NP_005746.2
• NCBI GenBank Nucleotide #: NM_005755.2
• UniProt Accession #: Q14213; O75269; A0N0N2
• Molecular Weight: 25,396 Da
• NCBI Official Full Name: interleukin-27 subunit beta
• NCBI Official Synonym Full Names: Epstein-Barr virus induced 3
• NCBI Official Symbol: EBI3
• NCBI Official Synonym Symbols: IL27B; IL-27B
• NCBI Protein Information: interleukin-27 subunit beta; IL27 subunit; IL35 subunit; cytokine receptor; IL-27 subunit beta; EBV-induced gene 3 protein; Epstein-Barr virus induced gene 3; epstein-Barr virus-induced gene 3 protein
• UniProt Protein Name: Interleukin-27 subunit beta
• Protein Family: Interleukin
• UniProt Gene Name: EBI3
• UniProt Synonym Gene Names: IL27B; IL-27 subunit beta; IL-27B; EBV-induced gene 3 protein
• UniProt Entry Name: IL27B_HUMAN

NCBI Description

This gene was identified by its induced expression in B lymphocytes in response Epstein-Barr virus infection. It encodes a secreted glycoprotein belonging to the hematopoietin receptor family, and heterodimerizes with a 28 kDa protein to form interleukin 27 (IL-27). IL-27 regulates T cell and inflammatory responses, in part by activating the Jak/STAT pathway of CD4+ T cells. [provided by RefSeq, Sep 2008]

Uniprot Description

IL27-beta: Cytokine with pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon- gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines. Belongs to the type I cytokine receptor family. Type 3 subfamily.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extracellular space; plasma membrane

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; interleukin-27 receptor binding; cytokine activity

Biological Process: T-helper 1 type immune response; cytokine and chemokine mediated signaling pathway; positive regulation of alpha-beta T cell proliferation; humoral immune response; positive regulation of interferon-gamma biosynthetic process