Western Blot (WB)
(A single >250 kDa band for the Hela wild type lysate was observed (3 ug/ml anti- FLNA) vs the predicted size of 280.7 kDa. The molecular weight discrepancy could be due a post-translationally modified form of the target protein, a splice-variant form of the target protein, or an unrelated protein which shares the antibody-reactive epitope. A weaker band of similar size was observed in the knock out lysate in the 20, 10, and 5 ug lanes, suggesting incomplete knockout of the target gene.)
Mouse FLNA Monoclonal Antibody | anti-FLNA antibody
FLNA Antibody [Knockout Validated]
WB: 1:4000
Western Blot (WB)
(A single >250 kDa band for the Hela wild type lysate was observed (3 ug/ml anti- FLNA) vs the predicted size of 280.7 kDa. The molecular weight discrepancy could be due a post-translationally modified form of the target protein, a splice-variant form of the target protein, or an unrelated protein which shares the antibody-reactive epitope. A weaker band of similar size was observed in the knock out lysate in the 20, 10, and 5 ug lanes, suggesting incomplete knockout of the target gene.)
Western Blot (WB)
(A single >250 kDa band for the Hela wild type lysate was observed (3 ug/ml anti- FLNA) vs the predicted size of 280.7 kDa. The molecular weight discrepancy could be due a post-translationally modified form of the target protein, a splice-variant form of the target protein, or an unrelated protein which shares the antibody-reactive epitope. A weaker band of similar size was observed in the knock out lysate in the 20, 10, and 5 ug lanes, suggesting incomplete knockout of the target gene.)
Immunofluorescence (IF)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling FLNA with AM2240B at 1/25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).)
Immunofluorescence (IF)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling FLNA with AM2240B at 1/25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).)
Western Blot (WB)
(All lanes : Anti-FLNA Antibody at 1:4000 dilutionLane 1: Hela whole cell lysateLane 2: C6 whole cell lysateLane 3: PC-3 whole cell lysateLane 4: COS-7 whole cell lysateLane 5: NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 280 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-FLNA Antibody at 1:4000 dilutionLane 1: Hela whole cell lysateLane 2: C6 whole cell lysateLane 3: PC-3 whole cell lysateLane 4: COS-7 whole cell lysateLane 5: NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 280 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]
Uniprot Description
FLNA: a ubiquitous cytoskeletal protein that promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Plays an essential role in embryonic cell migration. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interactions with filamin A may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking.
Protein type: Motility/polarity/chemotaxis; Actin-binding; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: Xq28
Cellular Component: cortical cytoskeleton; focal adhesion; extracellular region; actin filament; trans-Golgi network; cytosol; actin cytoskeleton; cell soma; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; dendritic shaft; nucleus
Molecular Function: Rho GTPase binding; protein homodimerization activity; Ral GTPase binding; Rac GTPase binding; transcription factor binding; small GTPase binding; actin filament binding; protein binding; signal transducer activity; protein kinase C binding; mu-type opioid receptor binding; glycoprotein binding; SMAD binding
Biological Process: actin crosslink formation; platelet activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein stabilization; negative regulation of transcription factor activity; receptor clustering; mRNA transcription from RNA polymerase II promoter; dopamine receptor, adenylate cyclase inhibiting pathway; establishment of protein localization; platelet degranulation; early endosome to late endosome transport; actin cytoskeleton reorganization; positive regulation of transcription factor import into nucleus; cytoplasmic sequestering of protein; epithelial to mesenchymal transition; cilium biogenesis; blood coagulation; negative regulation of protein catabolic process
Disease: Fg Syndrome 2; Terminal Osseous Dysplasia; Cardiac Valvular Dysplasia, X-linked; Frontometaphyseal Dysplasia; Melnick-needles Syndrome; Heterotopia, Periventricular, Ehlers-danlos Variant; Intestinal Pseudoobstruction, Neuronal, Chronic Idiopathic, X-linked; Heterotopia, Periventricular, X-linked Dominant; Otopalatodigital Syndrome, Type Ii; Otopalatodigital Syndrome, Type I
Research Articles on FLNA
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Product Notes
The FLNA flna (Catalog #AAA9232250) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The FLNA Antibody [Knockout Validated] reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's FLNA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). IF: 1:25 WB: 1:4000. Researchers should empirically determine the suitability of the FLNA flna for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FLNA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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