Western Blot (WB)
(All lanes : Anti-EEF1E1 Antibody at1:2000 dilutionLane 1: HepG2 whole cell lysateLane 2: A431 whole cell lysateLane 3: A549 whole cell lysateLane 4: Jurkat whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 20 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Mouse anti-Human EEF1E1 Monoclonal Antibody | anti-EEF1E1 antibody
EEF1E1 Antibody
FC/FACS: 1:25
IF: 1:25
Western Blot (WB)
(All lanes : Anti-EEF1E1 Antibody at1:2000 dilutionLane 1: HepG2 whole cell lysateLane 2: A431 whole cell lysateLane 3: A549 whole cell lysateLane 4: Jurkat whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 20 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-EEF1E1 Antibody at1:2000 dilutionLane 1: HepG2 whole cell lysateLane 2: A431 whole cell lysateLane 3: A549 whole cell lysateLane 4: Jurkat whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 20 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Flow Cytometry (FC/FACS)
(Overlay histogram showing Hela cells stained at 37 degree C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(NA168821)) at 1/400 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was mouse IgG2b (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Flow Cytometry (FC/FACS)
(Overlay histogram showing Hela cells stained at 37 degree C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(NA168821)) at 1/400 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was mouse IgG2b (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Immunofluorescence (IF)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).)
Immunofluorescence (IF)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).)
Background: Positive modulator of ATM response to DNA damage.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage, and it plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream MUTED (muted homolog) gene. An EEF1E1-related pseudogene has been identified on chromosome 2. [provided by RefSeq, Dec 2010]
Uniprot Description
EEF1E1: Positive modulator of ATM response to DNA damage.
Protein type: Translation; Translation elongation
Chromosomal Location of Human Ortholog: 6p24.3
Cellular Component: cytoplasm; nucleus; cytosol
Molecular Function: protein binding
Biological Process: negative regulation of cell proliferation; tRNA aminoacylation for protein translation; positive regulation of apoptosis; gene expression; positive regulation of DNA damage response, signal transduction by p53 class mediator
Research Articles on EEF1E1
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Product Notes
The EEF1E1 eef1e1 (Catalog #AAA9232191) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EEF1E1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EEF1E1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA). WB: 1:2000 FC/FACS: 1:25 IF: 1:25. Researchers should empirically determine the suitability of the EEF1E1 eef1e1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EEF1E1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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