Western Blot (WB)
(All lanes : Anti-PPM1H Antibody (Center) at 1:2000 dilutionLane 1: MCF-7 whole cell lysateLane 2: K562 whole cell lysateLane 3: mouse spleen lysateLane 4: Jurkat whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 56 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human, Mouse PPM1H Polyclonal Antibody | anti-PPM1H antibody
PPM1H Antibody (Center)
Gene Names
PPM1H; URCC2; ARHCL1; NERPP-2C
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
Synonyms
PPM1H; Polyclonal Antibody; PPM1H Antibody (Center); Protein phosphatase 1H; ARHCL1; KIAA1157; URCC2; anti-PPM1H antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Ig
Specificity
This PPM1H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-263 amino acids from the Central region of human PPM1H.
Sequence Length
514
Applicable Applications for anti-PPM1H antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes
WB: 1:2000
IHC-P: 1:50-100
FC/FACS: 1:10-50
IHC-P: 1:50-100
FC/FACS: 1:10-50
Antigen Source
Human
Antigen Region
235-263
Antigen Type
Synthesized Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(All lanes : Anti-PPM1H Antibody (Center) at 1:2000 dilutionLane 1: MCF-7 whole cell lysateLane 2: K562 whole cell lysateLane 3: mouse spleen lysateLane 4: Jurkat whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 56 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-PPM1H Antibody (Center) at 1:2000 dilutionLane 1: MCF-7 whole cell lysateLane 2: K562 whole cell lysateLane 3: mouse spleen lysateLane 4: Jurkat whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 56 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)-Paraffin
(Formalin-fixed and paraffin-embedded human brain tissue reacted with PPM1H Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)-Paraffin
(Formalin-fixed and paraffin-embedded human brain tissue reacted with PPM1H Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(PPM1H Antibody (Center) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(PPM1H Antibody (Center) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-PPM1H antibody
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
References
Sugiura,T., et.al., Cancer Biol. Ther. 7 (2), 285-292 (2008). Labes,M., et.al., Mol. Cell. Neurosci. 12 (1-2), 29-47 (1998).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
56448
NCBI Official Full Name
protein phosphatase 1H
NCBI Official Synonym Full Names
protein phosphatase, Mg2+/Mn2+ dependent 1H
NCBI Official Symbol
PPM1H
NCBI Official Synonym Symbols
URCC2; ARHCL1; NERPP-2C
NCBI Protein Information
protein phosphatase 1H
UniProt Protein Name
Protein phosphatase 1H
Protein Family
UniProt Gene Name
PPM1H
UniProt Synonym Gene Names
ARHCL1; KIAA1157; URCC2
UniProt Entry Name
PPM1H_HUMAN
Uniprot Description
PPM1H: Belongs to the PP2C family.
Protein type: Phosphatase; EC 3.1.3.16
Chromosomal Location of Human Ortholog: 12q14.1
Cellular Component: cytoplasm; nucleus
Molecular Function: phosphoprotein phosphatase activity
Biological Process: dephosphorylation
Research Articles on PPM1H
Similar Products
Product Notes
The PPM1H ppm1h (Catalog #AAA9232159) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPM1H Antibody (Center) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PPM1H can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), ELISA (EIA). WB: 1:2000 IHC-P: 1:50-100 FC/FACS: 1:10-50. Researchers should empirically determine the suitability of the PPM1H ppm1h for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPM1H, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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