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Western Blot (WB) (All lanes : Anti-HSPA9 Antibody (Center) at 1:2000 dilutionLane 1: PC-3 whole cell lysateLane 2: HepG2 whole cell lysateLane 3: Hela whole cell lysateLane 4: L929 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 74 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit HSPA9 Polyclonal Antibody | anti-HSPA9 antibody

HSPA9 Antibody (Center)

Gene Names
HSPA9; CSA; MOT; MOT2; SAAN; CRP40; EVPLS; GRP75; PBP74; GRP-75; HSPA9B; SIDBA4; MTHSP75; HEL-S-124m
Reactivity
Human, Mouse
Predicted: Bovine, Hamster, Rat
Applications
Western Blot, ELISA
Synonyms
HSPA9; Polyclonal Antibody; HSPA9 Antibody (Center); Stress-70 protein; mitochondrial; 75 kDa glucose-regulated protein; GRP-75; Heat shock 70 kDa protein 9; Mortalin; MOT; Peptide-binding protein 74; PBP74; GRP75; HSPA9B; mt-HSP70; anti-HSPA9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Predicted: Bovine, Hamster, Rat
Clonality
Polyclonal
Isotype
Ig
Specificity
This HSPA9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-301 amino acids from the Central region of human HSPA9.
Sequence Length
679
Applicable Applications for anti-HSPA9 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:2000
Antigen Source
Human
Antigen Region
273-301
Antigen Type
Synthesized Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-HSPA9 Antibody (Center) at 1:2000 dilutionLane 1: PC-3 whole cell lysateLane 2: HepG2 whole cell lysateLane 3: Hela whole cell lysateLane 4: L929 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 74 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-HSPA9 Antibody (Center) at 1:2000 dilutionLane 1: PC-3 whole cell lysateLane 2: HepG2 whole cell lysateLane 3: Hela whole cell lysateLane 4: L929 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 74 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-HSPA9 antibody
Description: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Background: This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.
Product Categories/Family for anti-HSPA9 antibody
References
Li, Y., et al. Environ. Health Perspect. 118(7):936-942(2010). Luo, W.I., et al. Protein Expr. Purif. 72(1):75-81(2010). Goswami, A.V., et al. J. Biol. Chem. 285(25):19472-19482(2010). Iosefson, O., et al. FEBS Lett. 584(6):1080-1084(2010). Rikova, K., et al. Cell 131(6):1190-1203(2007).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
73680
NCBI Official Full Name
Stress-70 protein, mitochondrial
NCBI Official Synonym Full Names
heat shock protein family A (Hsp70) member 9
NCBI Official Symbol
HSPA9
NCBI Official Synonym Symbols
CSA; MOT; MOT2; SAAN; CRP40; EVPLS; GRP75; PBP74; GRP-75; HSPA9B; SIDBA4; MTHSP75; HEL-S-124m
NCBI Protein Information
stress-70 protein, mitochondrial
UniProt Protein Name
Stress-70 protein, mitochondrial
Protein Family
UniProt Gene Name
HSPA9
UniProt Synonym Gene Names
GRP75; HSPA9B; mt-HSP70; GRP-75; MOT; PBP74
UniProt Entry Name
GRP75_HUMAN

NCBI Description

This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.[provided by RefSeq, May 2010]

Uniprot Description

HSPA9B: Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone. Interacts with FXN. Interacts with HSCB. Component of the MINOS/MitOS complex, that includes IMMT, HSPA9 and CHCHD3 and associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2. Belongs to the heat shock protein 70 family.

Protein type: Nucleolus; Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: focal adhesion; mitochondrion; cytoplasm; nucleolus

Molecular Function: protein binding; ubiquitin protein ligase binding; unfolded protein binding; ATP binding

Biological Process: cellular protein metabolic process; protein folding; protein export from nucleus; protein targeting to mitochondrion; negative regulation of apoptosis

Research Articles on HSPA9

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Product Notes

The HSPA9 hspa9 (Catalog #AAA9232154) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSPA9 Antibody (Center) reacts with Human, Mouse Predicted: Bovine, Hamster, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HSPA9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:2000. Researchers should empirically determine the suitability of the HSPA9 hspa9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSPA9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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