Immunohistochemistry (IHC)
(Staining Mouse Nkx2-5 in mouse spleen sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Rabbit anti-Mouse Nkx2-5 Polyclonal Antibody | anti-Nkx2-5 antibody
Mouse Nkx2-5 Antibody (Center)
Gene Names
Nkx2-5; Csx; Nkx2.5; tinman; Nkx-2.5
Reactivity
Mouse
Applications
Western Blot, ELISA
Synonyms
Nkx2-5; Polyclonal Antibody; Mouse Nkx2-5 Antibody (Center); Homeobox protein Nkx-25; Cardiac-specific homeobox; Homeobox protein CSX; Homeobox protein NK-2 homolog E; Csx; Nkx-25; Nkx2e; anti-Nkx2-5 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Ig
Specificity
This mouse Nkx2-5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 98-133 amino acids from the Central region of mouse Nkx2-5.
Sequence Length
318
Applicable Applications for anti-Nkx2-5 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
IHC: 1:25
WB: 1:2000
WB: 1:2000
Antigen Source
Human
Antigen Region
98-133
Antigen Type
Synthetic Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Immunohistochemistry (IHC)
(Staining Mouse Nkx2-5 in mouse spleen sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(Staining Mouse Nkx2-5 in mouse spleen sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Western Blot (WB)
(All lanes : Anti-Nkx2-5 Antibody (Center) at 1:2000 dilutionLane 1: mouse spleen lysatesLane 2: mouse stomach lysatesLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-Nkx2-5 Antibody (Center) at 1:2000 dilutionLane 1: mouse spleen lysatesLane 2: mouse stomach lysatesLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-Nkx2-5 antibody
Description: Purified Rabbit Polyclonal Antibody (Pab)
Background: Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4. It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B. Together with PBX1, it is required for spleen development through a mechanism that involves CDKN2B repression.
Background: Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4. It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B. Together with PBX1, it is required for spleen development through a mechanism that involves CDKN2B repression.
Product Categories/Family for anti-Nkx2-5 antibody
References
Lints T.J.,et al.Development 119:419-431(1993). Lints T.J.,et al.Development 119:969-969(1993). Searcy R.D.,et al.Development 125:4461-4470(1998). Komuro I.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:8145-8149(1993). Kim Y.H.,et al.J. Biol. Chem. 273:25875-25879(1998).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34163
NCBI Official Full Name
homeobox protein Nkx-2.5
NCBI Official Synonym Full Names
NK2 homeobox 5
NCBI Official Symbol
Nkx2-5
NCBI Official Synonym Symbols
Csx; Nkx2.5; tinman; Nkx-2.5
NCBI Protein Information
homeobox protein Nkx-2.5
UniProt Protein Name
Homeobox protein Nkx-2.5
Protein Family
UniProt Gene Name
Nkx2-5
UniProt Synonym Gene Names
Csx; Nkx-2.5; Nkx2e
UniProt Entry Name
NKX25_MOUSE
Research Articles on Nkx2-5
Similar Products
Product Notes
The Nkx2-5 nkx2-5 (Catalog #AAA9232073) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Mouse Nkx2-5 Antibody (Center) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Nkx2-5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). IHC: 1:25 WB: 1:2000. Researchers should empirically determine the suitability of the Nkx2-5 nkx2-5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nkx2-5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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