Western Blot (WB)
(All lanes : Anti-Nkx2-5 Antibody (Center) at 1:2000 dilutionLane 1: mouse stomach lysatesLane 2: mouse spleen lysatesLane 3: mouse heart lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 34 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Mouse Nkx2-5 Polyclonal Antibody | anti-Nkx2-5 antibody
Mouse Nkx2-5 Antibody (Center)
Western Blot (WB)
(All lanes : Anti-Nkx2-5 Antibody (Center) at 1:2000 dilutionLane 1: mouse stomach lysatesLane 2: mouse spleen lysatesLane 3: mouse heart lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 34 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-Nkx2-5 Antibody (Center) at 1:2000 dilutionLane 1: mouse stomach lysatesLane 2: mouse spleen lysatesLane 3: mouse heart lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 34 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-Nkx2-5 Antibody (Center) at 1:1000 dilutionLane 1: mouse spleen lysatesLane 2: mouse stomach lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 34 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-Nkx2-5 Antibody (Center) at 1:1000 dilutionLane 1: mouse spleen lysatesLane 2: mouse stomach lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 34 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Lints T.J.,et al.Development 119:969-969(1993).
Searcy R.D.,et al.Development 125:4461-4470(1998).
Komuro I.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:8145-8149(1993).
Kim Y.H.,et al.J. Biol. Chem. 273:25875-25879(1998).
NCBI and Uniprot Product Information
Uniprot Description
NKX2-5: Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4. Interacts with HIPK1 and HIPK2, but not HIPK3. Interacts with the C-terminal zinc finger of GATA4 through its homeobox domain. Also interacts with JARID2 which represses its ability to activate transcription of ANF. Interacts with FBLIM1. Expressed only in the heart. Belongs to the NK-2 homeobox family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding
Cellular Component: transcription factor complex; cytoplasm; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; histone deacetylase binding; chromatin binding; transcription factor binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; heart morphogenesis; tongue development; ventricular cardiac myofibril development; multicellular organismal development; positive regulation of transcription, DNA-dependent; heart development; ventricular cardiac muscle cell development; cardiac muscle cell proliferation; Wnt receptor signaling pathway through beta-catenin; negative regulation of transcription from RNA polymerase II promoter; cardiac muscle cell differentiation; BMP signaling pathway; regulation of transcription, DNA-dependent; atrial cardiac muscle cell development; cardiac muscle morphogensis; positive regulation of cell proliferation; thyroid gland development; hemopoiesis; regulation of cardiac muscle cell proliferation; heart looping; cell differentiation; vasculogenesis; cardiac muscle contraction; adult heart development; spleen development; cardiac muscle development; pharyngeal system development; sarcomere organization; regulation of transcription from RNA polymerase II promoter; heart contraction; positive regulation of cardioblast differentiation; embryonic heart tube development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; positive regulation of heart contraction; endoderm development; negative regulation of apoptosis
Research Articles on Nkx2-5
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Product Notes
The Nkx2-5 nkx2-5 (Catalog #AAA9204436) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Mouse Nkx2-5 Antibody (Center) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Nkx2-5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the Nkx2-5 nkx2-5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nkx2-5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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