Rabbit PRDX3 Polyclonal Antibody | anti-PRDX3 antibody

PRDX3 Antibody (C-term)

Cat. Num. AAA9231902

• Gene Names: PRDX3; AOP1; MER5; AOP-1; SP-22; HBC189; PRO1748; prx-III
• Reactivity: Human; Predicted: Mouse, Rat
• Applications: Western Blot, ELISA
• Synonyms: PRDX3; Polyclonal Antibody; PRDX3 Antibody (C-term); Thioredoxin-dependent peroxide reductase; mitochondrial; Antioxidant protein 1; AOP-1; HBC189; Peroxiredoxin III; Prx-III; Peroxiredoxin-3; Protein MER5 homolog; AOP1; anti-PRDX3 antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Mouse, Rat
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This PRDX3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 229-262 amino acids from the C-terminal region of human PRDX3.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 256

• Applicable Applications for anti-PRDX3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000
• Antigen Source: Human
• Antigen Region: 229-262
• Antigen Type: Synthetic Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of lysates from A431,Hela,MCF-7 cell line (from left to right), using PRDX3 Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)

Related Product Information for anti-PRDX3 antibody

Description: Purified Rabbit Polyclonal Antibody (Pab)

Background: Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical- generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.

References

Tsuji K.,et al.Biochem. J. 307:377-381(1995). Ota T.,et al.Nat. Genet. 36:40-45(2004). Ebert L.,et al.Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases. Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases. Deloukas P.,et al.Nature 429:375-381(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 2507171
• NCBI GeneID: 10935
• UniProt Accession #: P30048
• Molecular Weight: 27693
• NCBI Official Full Name: Thioredoxin-dependent peroxide reductase, mitochondrial
• NCBI Official Synonym Full Names: peroxiredoxin 3
• NCBI Official Symbol: PRDX3
• NCBI Official Synonym Symbols: AOP1; MER5; AOP-1; SP-22; HBC189; PRO1748; prx-III
• NCBI Protein Information: thioredoxin-dependent peroxide reductase, mitochondrial
• UniProt Protein Name: Thioredoxin-dependent peroxide reductase, mitochondrial
• Protein Family: Thioredoxin-dependent peroxide reductase
• UniProt Gene Name: PRDX3
• UniProt Synonym Gene Names: AOP1; AOP-1; Prx-III
• UniProt Entry Name: PRDX3_HUMAN

NCBI Description

This gene encodes a mitochondrial protein with antioxidant function. The protein is similar to the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase, and it can rescue bacterial resistance to alkylhydroperoxide in E. coli that lack the C22 subunit. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologs suggest that these genes consist of a family that is responsible for the regulation of cellular proliferation, differentiation and antioxidant functions. This family member can protect cells from oxidative stress, and it can promote cell survival in prostate cancer. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1, 3, 13 and 22. [provided by RefSeq, Oct 2014]

Uniprot Description

PRDX3: Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical- generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. Belongs to the AhpC/TSA family.

Protein type: Oxidoreductase; EC 1.11.1.15

Chromosomal Location of Human Ortholog: 10q25-q26

Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; early endosome; IkappaB kinase complex; cytosol

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; thioredoxin peroxidase activity; caspase inhibitor activity; alkyl hydroperoxide reductase activity; kinase binding; protein kinase binding

Biological Process: negative regulation of kinase activity; mitochondrion organization and biogenesis; response to lipopolysaccharide; negative regulation of caspase activity; activation of NF-kappaB transcription factor; response to reactive oxygen species; peptidyl-cysteine oxidation; response to hydrogen peroxide; regulation of mitochondrial membrane potential; hydrogen peroxide catabolic process; positive regulation of cell proliferation; myeloid cell differentiation; response to oxidative stress; negative regulation of apoptosis; maternal placenta development