NGEF blocking peptide

NGEF Blocking Peptide (C-term)

• Gene Names: NGEF; EPHEXIN; ARHGEF27
• Synonyms: NGEF; NGEF Blocking Peptide (C-term); Ephexin-1; Eph-interacting exchange protein; Neuronal guanine nucleotide exchange factor; NGEF blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 669-684 of HUMAN NGEF
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 710

• Cellular Location: Cytoplasm. Membrane. Cell projection, growth cone Note: Associated with membranes. Localizes to axonal growth cones (By similarity).
• Tissue Location: Highly expressed in brain specifically in caudate nucleus and to a lower extent in amygdala and hippocampus Also detected in lung.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for NGEF blocking peptide

Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 114152090
• NCBI GeneID: 25791
• UniProt Accession #: Q8N5V2; Q53QQ4; Q53ST7; Q6GMQ5; Q9NQD6; B4DMB8; B9A045; E9PC42
• Molecular Weight: 71,387 Da
• NCBI Official Full Name: Ephexin-1
• NCBI Official Synonym Full Names: neuronal guanine nucleotide exchange factor
• NCBI Official Symbol: NGEF
• NCBI Official Synonym Symbols: EPHEXIN; ARHGEF27
• NCBI Protein Information: ephexin-1
• UniProt Protein Name: Ephexin-1
• Protein Family: Ephexin
• UniProt Gene Name: NGEF
• UniProt Entry Name: NGEF_HUMAN

Uniprot Description

ephexin-1: Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension. Interacts with CDK5R1 and EPHA4; activated by EPHA4 through the CDK5 kinase. Highly expressed in brain specifically in caudate nucleus and to a lower extent in amygdala and hippocampus. Also detected in lung. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs, Rac/Rho; GEFs

Chromosomal Location of Human Ortholog: 2q37

Cellular Component: cytosol

Molecular Function: guanyl-nucleotide exchange factor activity; Rho guanyl-nucleotide exchange factor activity

Biological Process: ephrin receptor signaling pathway; positive regulation of apoptosis; regulation of GTPase activity; regulation of small GTPase mediated signal transduction