Uhrf1 blocking peptide

Uhrf1 Blocking Peptide (C-term)

• Gene Names: Uhrf1; Np95; ICBP90; RNF106; AL022808
• Synonyms: Uhrf1; Uhrf1 Blocking Peptide (C-term); E3 ubiquitin-protein ligase UHRF1; 632-; Nuclear protein 95; Nuclear zinc finger protein Np95; Ubiquitin-like PHD and RING finger domain-containing protein 1; mUhrf1; Ubiquitin-like-containing PHD and RING finger domains protein 1; Np95; Uhrf1 blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 596-608 of HUMAN Uhrf1
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 782

• Cellular Location: Nucleus. Note: Localizes to replication foci Enriched in pericentric heterochromatin. Also localizes to euchromatic regions
• Tissue Location: Expressed in thymus, testis, spleen and lung. Within testis, expressed in almost all cells except elongated spermatids.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Uhrf1 blocking peptide

Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD- type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. May be involved in DNA repair.

NCBI and Uniprot Product Information

• NCBI GI #: 67462071
• NCBI GeneID: 18140
• UniProt Accession #: Q8VDF2; Q3U9D7; Q3U9P2; Q3UI74; Q3UIE6; Q3ULF2; Q3ULQ0; Q8C6F1; Q8VIA1; Q9Z1H6
• Molecular Weight: 87,556 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase UHRF1
• NCBI Official Synonym Full Names: ubiquitin-like, containing PHD and RING finger domains, 1
• NCBI Official Symbol: Uhrf1
• NCBI Official Synonym Symbols: Np95; ICBP90; RNF106; AL022808
• NCBI Protein Information: E3 ubiquitin-protein ligase UHRF1
• UniProt Protein Name: E3 ubiquitin-protein ligase UHRF1
• Protein Family: UHRF1-binding protein
• UniProt Gene Name: Uhrf1
• UniProt Synonym Gene Names: Np95; mUhrf1
• UniProt Entry Name: UHRF1_MOUSE

Uniprot Description

UHRF1: Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD- type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. May be involved in DNA repair. Defects in UHRF1 may be a cause of cancers. Overexpressed in many different forms of human cancers, including bladder, breast, cervical, colorectal and prostate cancers, as well as pancreatic adenocarcinomas, rhabdomyosarcomas and gliomas. Plays an important role in the correlation of histone modification and gene silencing in cancer progression. Expression is associated with a poor prognosis in patients with various cancers, suggesting that it participates in cancer progression. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; EC 6.3.2.-; Ubiquitin ligase; Ubiquitin conjugating system; Transcription factor; EC 6.3.2.19; DNA-binding

Cellular Component: cytoplasmic vesicle; euchromatin; heterochromatin; nuclear chromatin; nuclear heterochromatin; nuclear matrix; nucleus; plasma membrane; replication fork

Molecular Function: histone binding; identical protein binding; methyl-CpG binding; methylated histone residue binding; nucleosomal histone binding; protein binding; ubiquitin-protein ligase activity; zinc ion binding

Biological Process: cell proliferation; histone monoubiquitination; histone ubiquitination; maintenance of DNA methylation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cellular protein metabolic process; protein autoubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process