PIP4K2A blocking peptide

PIP4K2A Blocking Peptide (C-term)

• Gene Names: PIP4K2A; PIPK; PI5P4KA; PIP5K2A; PIP5KIIA; PIP5KII-alpha
• Synonyms: PIP4K2A; PIP4K2A Blocking Peptide (C-term); Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha; 1-phosphatidylinositol 5-phosphate 4-kinase 2-alpha; Diphosphoinositide kinase 2-alpha; PIP5KIII; Phosphatidylinositol 5-phosphate 4-kinase type II alpha; PI(5)P 4-kinase type II alpha; PIP4KII-alpha; PtdIns(4)P-5-kinase B isoform; PtdIns(4)P-5-kinase C isoform; PtdIns(5)P-4-kinase isoform 2-alpha; PIP5K2; PIP5K2A; PIP4K2A blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 329-343 of HUMAN PIP4K2A
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 406

• Cellular Location: Cell membrane. Nucleus. Cytoplasm. Note: May translocate from the cytosol to the cell membrane upon activation of tyrosine phosphorylation. May translocate from the inner to the outer segments of the rod photoreceptor cells in response to light (By similarity). Localization to the nucleus is modulated by the interaction with PIP4K2B.
• Tissue Location: Expressed ubiquitously, with high levels in the brain. Present in most tissues, except notably skeletal muscle and small intestine.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for PIP4K2A blocking peptide

Catalyzes the phosphorylation of phosphatidylinositol 5- phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. May regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation. May negatively regulate insulin- stimulated glucose uptake by lowering the levels of PtdIns5P. May be involved in thrombopoiesis, and the terminal maturation of megakaryocytes and regulation of their size.

NCBI and Uniprot Product Information

• NCBI GI #: 18266879
• NCBI GeneID: 5305
• UniProt Accession #: P48426; P53807; Q5VUX3; B0YJ66; B4DGX2; D3DRV1
• Molecular Weight: 39,802 Da
• NCBI Official Full Name: Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha
• NCBI Official Synonym Full Names: phosphatidylinositol-5-phosphate 4-kinase type 2 alpha
• NCBI Official Symbol: PIP4K2A
• NCBI Official Synonym Symbols: PIPK; PI5P4KA; PIP5K2A; PIP5KIIA; PIP5KII-alpha
• NCBI Protein Information: phosphatidylinositol 5-phosphate 4-kinase type-2 alpha
• UniProt Protein Name: Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha
• Protein Family: Phosphatidylinositol 5-phosphate 4-kinase
• UniProt Gene Name: PIP4K2A
• UniProt Synonym Gene Names: PIP5K2; PIP5K2A; PI(5)P 4-kinase type II alpha; PIP4KII-alpha
• UniProt Entry Name: PI42A_HUMAN

NCBI Description

Phosphatidylinositol-5,4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. [provided by RefSeq, Jul 2008]

Uniprot Description

PIP4K2A: Catalyzes the phosphorylation of phosphatidylinositol 5- phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. May regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation. May negatively regulate insulin- stimulated glucose uptake by lowering the levels of PtdIns5P. May be involved in thrombopoiesis, and the terminal maturation of megakaryocytes and regulation of their size.

Protein type: EC 2.7.1.149; Carbohydrate Metabolism - inositol phosphate; Motility/polarity/chemotaxis; Kinase, lipid

Chromosomal Location of Human Ortholog: 10p12.2

Cellular Component: autophagic vacuole; cytosol

Molecular Function: 1-phosphatidylinositol-5-phosphate 4-kinase activity

Biological Process: phosphatidylinositol biosynthetic process; regulation of autophagy; regulation of phosphoinositide 3-kinase cascade