Lyn blocking peptide

Mouse Lyn Blocking Peptide (Center)

• Gene Names: Lyn; Hck-2; p53Lyn; p56Lyn; AA407514
• Synonyms: Lyn; Mouse Lyn Blocking Peptide (Center); Tyrosine-protein kinase Lyn; V-yes-1 Yamaguchi sarcoma viral related oncogene homolog; p53Lyn; p56Lyn; Lyn blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 229-243 of HUMAN Lyn
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 512

• Cellular Location: Cell membrane. Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Golgi apparatus Note: Accumulates in the nucleus by inhibition of Crm1-mediated nuclear export. Nuclear accumulation is increased by inhibition of its kinase activity. The trafficking from the Golgi apparatus to the cell membrane occurs in a kinase domain-dependent but kinase activity independent manner and is mediated by exocytic vesicular transport (By similarity).
• Tissue Location: Detected in bone marrow-derived monocytes (at protein level). Expressed predominantly in B-lymphoid and myeloid cells.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Lyn blocking peptide

Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. Functions primarily as negative regulator, but can also function as activator, depending on the context. Required for the initiation of the B-cell response, but also for its down- regulation and termination. Plays an important role in the regulation of B-cell differentiation, proliferation, survival and apoptosis, and is important for immune self-tolerance. Acts downstream of several immune receptors, including the B-cell receptor, CD79A, CD79B, CD5, CD19, CD22, FCER1, FCGR2, FCGR1A, TLR2 and TLR4. Plays a role in the inflammatory response to bacterial lipopolysaccharide. Mediates the responses to cytokines and growth factors in hematopoietic progenitors, platelets, erythrocytes, and in mature myeloid cells, such as dendritic cells, neutrophils and eosinophils. Acts downstream of EPOR, KIT, MPL, the chemokine receptor CXCR4, as well as the receptors for IL3, IL5 and CSF2. Plays an important role in integrin signaling. Regulates cell proliferation, survival, differentiation, migration, adhesion, degranulation, and cytokine release. Down- regulates signaling pathways by phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIM), that then serve as binding sites for phosphatases, such as PTPN6/SHP-1, PTPN11/SHP-2 and INPP5D/SHIP-1, that modulate signaling by dephosphorylation of kinases and their substrates. Phosphorylates LIME1 in response to CD22 activation. Phosphorylates BTK, CBL, CD5, CD19, CD72, CD79A, CD79B, CSF2RB, DOK1, HCLS1, LILRB3/PIR-B, MS4A2/FCER1B, PTK2B/PYK2, SYK and TEC. Promotes phosphorylation of SIRPA, PTPN6/SHP-1, PTPN11/SHP-2 and INPP5D/SHIP-1. Required for rapid phosphorylation of FER in response to FCER1 activation. Mediates KIT phosphorylation. Acts as an effector of EPOR (erythropoietin receptor) in controlling KIT expression and may play a role in erythroid differentiation during the switch between proliferation and maturation. Depending on the context, activates or inhibits several signaling cascades. Regulates phosphatidylinositol 3- kinase activity and AKT1 activation. Regulates activation of the MAP kinase signaling cascade, including activation of MAP2K1/MEK1, MAPK1/ERK2, MAPK3/ERK1, MAPK8/JNK1 and MAPK9/JNK2. Mediates activation of STAT5A and/or STAT5B. Phosphorylates LPXN on 'Tyr- 72'. Kinase activity facilitates TLR4-TLR6 heterodimerization and signal initiation.

NCBI and Uniprot Product Information

• NCBI GI #: 2507208
• NCBI GeneID: 17096
• UniProt Accession #: P25911; Q62127
• Molecular Weight: 56,285 Da
• NCBI Official Full Name: Tyrosine-protein kinase Lyn
• NCBI Official Synonym Full Names: LYN proto-oncogene, Src family tyrosine kinase
• NCBI Official Symbol: Lyn
• NCBI Official Synonym Symbols: Hck-2; p53Lyn; p56Lyn; AA407514
• NCBI Protein Information: tyrosine-protein kinase Lyn
• UniProt Protein Name: Tyrosine-protein kinase Lyn
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Lyn
• UniProt Entry Name: LYN_MOUSE

Uniprot Description

LYN: a tyrosine kinase of the Src family that plays a role in B cell antigen-receptor signaling.

Protein type: Kinase, protein; Protein kinase, TK; Oncoprotein; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; TK group; Src family

Cellular Component: cell-cell adherens junction; cytoplasm; extrinsic to internal side of plasma membrane; Golgi apparatus; lipid raft; membrane; mitochondrial crista; mitochondrial intermembrane space; mitochondrial membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; postsynaptic density

Molecular Function: enzyme binding; erythropoietin receptor binding; gamma-tubulin binding; glycosphingolipid binding; integrin binding; non-membrane spanning protein tyrosine kinase activity; phosphoprotein binding; platelet-derived growth factor receptor binding; protein binding; protein complex binding; protein kinase activity; protein-tyrosine kinase activity; receptor binding; SH3 domain binding; ubiquitin protein ligase binding

Biological Process: B cell homeostasis; B cell receptor signaling pathway; central nervous system development; chemotaxis; cytokine secretion; erythrocyte differentiation; Fc receptor mediated inhibitory signaling pathway; Fc receptor mediated stimulatory signaling pathway; hemoglobin biosynthetic process; hemopoiesis; histamine secretion by mast cell; immune response-regulating cell surface receptor signaling pathway; lipopolysaccharide-mediated signaling pathway; negative regulation of B cell proliferation; negative regulation of cell proliferation; negative regulation of MAP kinase activity; negative regulation of myeloid leukocyte differentiation; negative regulation of protein amino acid phosphorylation; negative regulation of toll-like receptor 2 signaling pathway; negative regulation of toll-like receptor 4 signaling pathway; neurite development; peptidyl-tyrosine phosphorylation; platelet degranulation; positive regulation of B cell receptor signaling pathway; positive regulation of cell migration; positive regulation of cell motility; positive regulation of cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of phosphorylation; positive regulation of tyrosine phosphorylation of STAT protein; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of B cell apoptosis; regulation of B cell receptor signaling pathway; regulation of cell adhesion mediated by integrin; regulation of cytokine production; regulation of cytokine secretion; regulation of erythrocyte differentiation; regulation of inflammatory response; regulation of mast cell activation; regulation of mast cell degranulation; regulation of release of sequestered calcium ion into cytosol; response to DNA damage stimulus; response to hormone stimulus; signal transduction; tolerance induction to self antigen; transmembrane receptor protein tyrosine kinase signaling pathway