NUP153 blocking peptide

NUP153 Antibody (N-term) Blocking Peptide

• Gene Names: NUP153; N153; HNUP153
• Synonyms: NUP153; NUP153 Antibody (N-term) Blocking Peptide; Nuclear pore complex protein Nup153; 153 kDa nucleoporin; Nucleoporin Nup153; NUP153 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human NUP153. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 1475

• Cellular Location: Nucleus. Nucleus membrane. Nucleus, nuclear pore complex. Note: Tightly associated with the nuclear membrane and lamina (By similarity). Localized to the nucleoplasmic side of the nuclear pore complex (NPC) core structure, forming a fibrous structure called the nuclear basket. Dissociates from the NPC structure early during prophase of mitosis. Integrated in the newly assembled nuclear envelope of postmitotic cells early in G1 Colocalized with NUP98 and TPR to the nuclear basket at the nucleoplasmic side of the NPC. Detected in diffuse and discrete intranuclear foci. Remained localized to the nuclear membrane after poliovirus (PV) infection.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for NUP153 blocking peptide

Component of the nuclear pore complex (NPC), a complex required for the trafficking across the nuclear envelope. Functions as a scaffolding element in the nuclear phase of the NPC essential for normal nucleocytoplasmic transport of proteins and mRNAs. Involved in the quality control and retention of unspliced mRNAs in the nucleus; in association with TPR, regulates the nuclear export of unspliced mRNA species bearing constitutive transport element (CTE) in a NXF1- and KHDRBS1-independent manner. Mediates TPR anchoring to the nuclear membrane at NPC. The repeat- containing domain may be involved in anchoring other components of the NPC to the pore membrane. Possible DNA-binding subunit of the nuclear pore complex (NPC).

NCBI and Uniprot Product Information

• NCBI GI #: 206729891
• NCBI GeneID: 9972
• UniProt Accession #: P49790; Q4LE47; Q5T9I7; Q7Z743; B4DIK2; E7EPX5; F6QR24
• Molecular Weight: 157,338 Da
• NCBI Official Full Name: Nuclear pore complex protein Nup153
• NCBI Official Synonym Full Names: nucleoporin 153
• NCBI Official Symbol: NUP153
• NCBI Official Synonym Symbols: N153; HNUP153
• NCBI Protein Information: nuclear pore complex protein Nup153
• UniProt Protein Name: Nuclear pore complex protein Nup153
• Protein Family: Nuclear pore complex protein
• UniProt Gene Name: NUP153
• UniProt Entry Name: NU153_HUMAN

NCBI Description

Nuclear pore complexes regulate the transport of macromolecules between the nucleus and cytoplasm. They are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are glycoproteins found in nuclear pores and contain characteristic pentapeptide XFXFG repeats as well as O-linked N-acetylglucosamine residues oriented towards the cytoplasm. The protein encoded by this gene has three distinct domains: a N-terminal region containing a pore targeting and an RNA-binding domain domain, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]

Uniprot Description

NUP153: Possible DNA-binding subunit of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane.

Protein type: Nuclear export; Nucleoporin

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: cytoplasm; nuclear inclusion body; nuclear membrane; nuclear pore; nucleolus; nucleoplasm

Molecular Function: identical protein binding; nucleocytoplasmic transporter activity; protein anchor; protein binding; structural constituent of nuclear pore

Biological Process: mitotic nuclear envelope disassembly; mRNA export from nucleus; negative regulation of RNA export from nucleus; nuclear pore complex assembly; protein sumoylation; RNA-mediated gene silencing; tRNA export from nucleus; viral reproduction; viral transcription