GDNF blocking peptide

GDNF Antibody (N-term) Blocking Peptide

• Gene Names: GDNF; ATF1; ATF2; HSCR3; HFB1-GDNF
• Synonyms: GDNF; GDNF Antibody (N-term) Blocking Peptide; Glial cell line-derived neurotrophic factor; hGDNF; Astrocyte-derived trophic factor; ATF; GDNF blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human GDNF. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 211

• Cellular Location: Secreted.
• Tissue Location: In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for GDNF blocking peptide

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

NCBI and Uniprot Product Information

• NCBI GI #: 729567
• NCBI GeneID: 2668
• UniProt Accession #: P39905; O95448; O95449; O95986; Q6FH33; Q96L44; Q9UD32; Q9UD33; Q9UMV2; Q9UP67; Q9UP97; B7WPK7; AAB33493; AAB33494
• Molecular Weight: 18,123 Da
• NCBI Official Full Name: Glial cell line-derived neurotrophic factor
• NCBI Official Synonym Full Names: glial cell derived neurotrophic factor
• NCBI Official Symbol: GDNF
• NCBI Official Synonym Symbols: ATF1; ATF2; HSCR3; HFB1-GDNF
• NCBI Protein Information: glial cell line-derived neurotrophic factor
• UniProt Protein Name: Glial cell line-derived neurotrophic factor
• Protein Family: GDNF family receptor
• UniProt Gene Name: GDNF
• UniProt Synonym Gene Names: hGDNF; ATF
• UniProt Entry Name: GDNF_HUMAN

NCBI Description

This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene may be associated with Hirschsprung disease. [provided by RefSeq, Jun 2010]

Uniprot Description

GDNF: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Defects in GDNF may be a cause of Hirschsprung disease type 3 (HSCR3). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. Belongs to the TGF-beta family. GDNF subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5p13.1-p12

Cellular Component: extracellular region

Molecular Function: protein binding; protein homodimerization activity; Ras guanyl-nucleotide exchange factor activity; receptor binding

Biological Process: adult locomotory behavior; enteric nervous system development; MAPKKK cascade; metanephros development; mRNA stabilization; negative regulation of apoptosis; negative regulation of neuron apoptosis; nervous system development; neural crest cell migration; neurite development; peristalsis; positive regulation of cell differentiation; positive regulation of cell proliferation; positive regulation of dopamine secretion; positive regulation of monooxygenase activity; positive regulation of transcription from RNA polymerase II promoter; postganglionic parasympathetic nervous system development; regulation of dopamine uptake; regulation of gene expression; signal transduction; sympathetic nervous system development; ureteric bud branching

Disease: Central Hypoventilation Syndrome, Congenital; Hirschsprung Disease, Susceptibility To, 3; Pheochromocytoma