NEK2 blocking peptide

NEK2 Antibody (Center) Blocking Peptide

• Gene Names: NEK2; NLK1; RP67; NEK2A; HsPK21; PPP1R111
• Synonyms: NEK2; NEK2 Antibody (Center) Blocking Peptide; Serine/threonine-protein kinase Nek2; HSPK 21; Never in mitosis A-related kinase 2; NimA-related protein kinase 2; NimA-like protein kinase 1; NEK2A; NLK1; NEK2 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the Center region of human NEK2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 445

• Cellular Location: Isoform 1: Nucleus. Nucleus, nucleolus. Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Chromosome, centromere, kinetochore. Chromosome, centromere Note: STK3/MST2 and SAV1 are required for its targeting to the centrosome. Colocalizes with SGOL1 and MAD1L1 at the kinetochore Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end Isoform 4: Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note: Predominantly nuclear
• Tissue Location: Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types. Isoform 1 and isoform 4 are expressed in the testis. Up- regulated in various cancer cell lines, as well as primary breast tumors.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for NEK2 blocking peptide

Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGOL1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Isoform 1 phosphorylates and activates NEK11 in G1/S- arrested cells. Isoform 2, which is not present in the nucleolus, does not.

NCBI and Uniprot Product Information

• NCBI GI #: 1709252
• NCBI GeneID: 4751
• UniProt Accession #: P51955; Q53FD6; Q5I1Z9; Q5VXZ1; Q6NZX8; Q7Z634; Q86XH2; Q96QN9
• Molecular Weight: 50,909 Da
• NCBI Official Full Name: Serine/threonine-protein kinase Nek2
• NCBI Official Synonym Full Names: NIMA related kinase 2
• NCBI Official Symbol: NEK2
• NCBI Official Synonym Symbols: NLK1; RP67; NEK2A; HsPK21; PPP1R111
• NCBI Protein Information: serine/threonine-protein kinase Nek2
• UniProt Protein Name: Serine/threonine-protein kinase Nek2
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: NEK2
• UniProt Synonym Gene Names: NEK2A; NLK1; NimA-related protein kinase 2
• UniProt Entry Name: NEK2_HUMAN

NCBI Description

This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

NEK2: a protein kinase of the NEK family. Accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Phosphorylation of Hec1 by Nek2 is essential for faithful chromosome segregation

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Nucleolus; Other group; NEK family

Chromosomal Location of Human Ortholog: 1q32.3

Cellular Component: centrosome; cytoplasm; cytosol; kinetochore; nucleus; protein complex

Molecular Function: protein binding; protein kinase activity; protein phosphatase binding; protein serine/threonine kinase activity

Biological Process: centrosome separation; chromosome segregation; G2/M transition of mitotic cell cycle; mitosis; positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of attachment of spindle microtubules to kinetochore; regulation of mitosis; regulation of mitotic centrosome separation; spindle assembly

Disease: Retinitis Pigmentosa 67