PKD2 Blocking Peptide | PRKD2 blocking peptide

PKD2 Antibody (C-term) Blocking Peptide

• Gene Names: PRKD2; PKD2; HSPC187; nPKC-D2
• Synonyms: PKD2; PKD2 Antibody (C-term) Blocking Peptide; Serine/threonine-protein kinase D2; nPKC-D2; PRKD2; PRKD2 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human PKD2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 878

• Cellular Location: Cytoplasm. Cell membrane. Nucleus. Golgi apparatus, trans-Golgi network. Note: Translocation to the cell membrane is required for kinase activation (By similarity). Accumulates in the nucleus upon CK1-mediated phosphorylation after activation of G-protein-coupled receptors Nuclear accumulation is regulated by blocking nuclear export of active PRKD2 rather than by increasing import.
• Tissue Location: Widely expressed.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for PRKD2 blocking peptide

Serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, and is involved in the regulation of cell proliferation via MAPK1/3 (ERK1/2) signaling, oxidative stress- induced NF-kappa-B activation, inhibition of HDAC7 transcriptional repression, signaling downstream of T-cell antigen receptor (TCR) and cytokine production, and plays a role in Golgi membrane trafficking, angiogenesis, secretory granule release and cell adhesion. May potentiate mitogenesis induced by the neuropeptide bombesin by mediating an increase in the duration of MAPK1/3 (ERK1/2) signaling, which leads to accumulation of immediate-early gene products including FOS that stimulate cell cycle progression. In response to oxidative stress, is phosphorylated at Tyr-438 by ABL1, which leads to the activation of PRKD2 without increasing its catalytic activity, and mediates activation of NF-kappa-B. In response to the activation of the gastrin receptor CCKBR, is phosphorylated at Ser-244 by CSNK1D and CSNK1E, translocates to the nucleus, phosphorylates HDAC7, leading to nuclear export of HDAC7 and inhibition of HDAC7 transcriptional repression of NR4A1/NUR77. Upon TCR stimulation, is activated independently of ZAP70, translocates from the cytoplasm to the nucleus and is required for interleukin-2 (IL2) promoter up-regulation. During adaptive immune responses, is required in peripheral T-lymphocytes for the production of the effector cytokines IL2 and IFNG after TCR engagement and for optimal induction of antibody responses to antigens. In epithelial cells stimulated with lysophosphatidic acid (LPA), is activated through a PKC-dependent pathway and mediates LPA-stimulated interleukin-8 (IL8) secretion via a NF- kappa-B-dependent pathway. During TCR-induced T-cell activation, interacts with and is activated by the tyrosine kinase LCK, which results in the activation of the NFAT transcription factors. In the trans-Golgi network (TGN), regulates the fission of transport vesicles that are on their way to the plasma membrane and in polarized cells is involved in the transport of proteins from the TGN to the basolateral membrane. Plays an important role in endothelial cell proliferation and migration prior to angiogenesis, partly through modulation of the expression of KDR/VEGFR2 and FGFR1, two key growth factor receptors involved in angiogenesis. In secretory pathway, is required for the release of chromogranin-A (CHGA)-containing secretory granules from the TGN. Downstream of PRKCA, plays important roles in angiotensin-2- induced monocyte adhesion to endothelial cells. Plays a regulatory role in angiogenesis and tumor growth by phosphorylating a downstream mediator CIB1 isoform 2, resulting in vascular endothelial growth factor A (VEGFA) secretion.

NCBI and Uniprot Product Information

• NCBI GI #: 296434570
• NCBI GeneID: 25865
• UniProt Accession #: Q9BZL6; Q8NCK8; Q8TB08; Q9P0T6; Q9Y3X8; M0R2R2
• Molecular Weight: 80,143 Da
• NCBI Official Full Name: Serine/threonine-protein kinase D2
• NCBI Official Synonym Full Names: protein kinase D2
• NCBI Official Symbol: PRKD2
• NCBI Official Synonym Symbols: PKD2; HSPC187; nPKC-D2
• NCBI Protein Information: serine/threonine-protein kinase D2
• UniProt Protein Name: Serine/threonine-protein kinase D2
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PRKD2
• UniProt Synonym Gene Names: PKD2
• UniProt Entry Name: KPCD2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

PRKD2: a calcium-independent, phospholipid-dependent CAMK kinase of the PKD family. Activated by diacylglycerol and phorbol esters. may play a role in carcinoid tumor progression.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; EC 2.7.11.13; Motility/polarity/chemotaxis; CAMK group; PKD family

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: protein binding; protein kinase activity; protein serine/threonine kinase activity

Biological Process: activation of CREB transcription factor; activation of NF-kappaB transcription factor; cell death; peptidyl-serine phosphorylation; positive regulation of angiogenesis; positive regulation of blood vessel endothelial cell migration; positive regulation of cell adhesion; positive regulation of endothelial cell proliferation; positive regulation of fibroblast growth factor receptor signaling pathway; positive regulation of interleukin-2 production; positive regulation of interleukin-8 production; positive regulation of peptidyl-serine phosphorylation; positive regulation of T cell receptor signaling pathway; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of vascular endothelial growth factor receptor signaling pathway; protein amino acid autophosphorylation; protein amino acid phosphorylation; T cell receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway