RYK blocking peptide

RYK Antibody (Center C397) Blocking Peptide

• Gene Names: RYK; JTK5; RYK1; JTK5A; D3S3195
• Synonyms: RYK; RYK Antibody (Center C397) Blocking Peptide; Tyrosine-protein kinase RYK; JTK5A; RYK blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the Center region of human RYK. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 604

• Cellular Location: Membrane; Single-pass type I membrane protein. Nucleus. Cytoplasm. Note: In cells that have undergone neuronal differentiation, the C-terminal cleaved part is translocated from the cytoplasm to the nucleus.
• Tissue Location: Observed in all the tissues examined.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for RYK blocking peptide

May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C- terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development.

NCBI and Uniprot Product Information

• NCBI GI #: 1710811
• NCBI GeneID: 6259
• UniProt Accession #: P34925; Q04696
• Molecular Weight: 67,764 Da
• NCBI Official Full Name: Tyrosine-protein kinase RYK
• NCBI Official Synonym Full Names: receptor-like tyrosine kinase
• NCBI Official Symbol: RYK
• NCBI Official Synonym Symbols: JTK5; RYK1; JTK5A; D3S3195
• NCBI Protein Information: tyrosine-protein kinase RYK
• UniProt Protein Name: Tyrosine-protein kinase RYK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: RYK
• UniProt Synonym Gene Names: JTK5A
• UniProt Entry Name: RYK_HUMAN

NCBI Description

The protein encoded by this gene is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. The encoded protein has a leucine-rich extracellular domain with a WIF-type Wnt binding region, a single transmembrane domain, and an intracellular tyrosine kinase domain. This protein is involved in stimulating Wnt signaling pathways such as the regulation of axon pathfinding. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]

Uniprot Description

RYK: a receptor tyrosine kinase of the Ryk family. Associates with, and may modulate the signaling of, EphB2 and EphB3. Expressed in nestin-positive progenitor cells and neurons, some oligodendrocytes, O-2A progenitor cells, and type-2 astrocytes in developing CNS.

Protein type: Kinase, protein; Membrane protein, integral; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Protein kinase, TK; TK group; Ryk family

Chromosomal Location of Human Ortholog: 3q22

Cellular Component: cytoplasm; integral to plasma membrane; membrane; nucleus; plasma membrane

Molecular Function: frizzled binding; protein binding; protein-tyrosine kinase activity; transmembrane receptor activity; transmembrane receptor protein tyrosine kinase activity; Wnt receptor activity; Wnt-protein binding

Biological Process: axon guidance; axonogenesis; cell proliferation in midbrain; corpus callosum development; neurite development; neuron differentiation; positive regulation of MAPKKK cascade; protein amino acid phosphorylation; signal transduction; Wnt receptor signaling pathway