PRAK (MAPKAPK5) (T182) Blocking Peptide | MAPKAPK5 blocking peptide

PRAK (MAPKAPK5) Antibody (T182) Blocking peptide

• Gene Names: MAPKAPK5; MK5; MK-5; PRAK; MAPKAP-K5
• Synonyms: PRAK (MAPKAPK5) (T182); PRAK (MAPKAPK5) Antibody (T182) Blocking peptide; MAP kinase-activated protein kinase 5; MAPK-activated protein kinase 5; MAPKAP kinase 5; MAPKAP-K5; MAPKAPK-5; MK-5; MK5; p38-regulated/activated protein kinase; PRAK; MAPKAPK5; MAPKAPK5 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the T182 region of human MAPKAPK5. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 473

• Cellular Location: Cytoplasm. Nucleus. Note: Translocates to the cytoplasm following phosphorylation and activation. Interaction with ERK3/MAPK6 or ERK4/MAPK4 and phosphorylation at Thr-182, activates the protein kinase activity, followed by translocation to the cytoplasm. Phosphorylation by PKA/PRKACA at Ser-115 also induces nuclear export
• Tissue Location: Expressed ubiquitously.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for MAPKAPK5 blocking peptide

Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras- induced senescence and phosphorylating p53/TP53. Involved in post- transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement.

NCBI and Uniprot Product Information

• NCBI GI #: 52000829
• NCBI GeneID: 8550
• UniProt Accession #: Q8IW41; O60491; Q86X46; Q9BVX9; Q9UG86; B3KVA5
• Molecular Weight: 54,035 Da
• NCBI Official Full Name: MAP kinase-activated protein kinase 5
• NCBI Official Synonym Full Names: mitogen-activated protein kinase-activated protein kinase 5
• NCBI Official Symbol: MAPKAPK5
• NCBI Official Synonym Symbols: MK5; MK-5; PRAK; MAPKAP-K5
• NCBI Protein Information: MAP kinase-activated protein kinase 5
• UniProt Protein Name: MAP kinase-activated protein kinase 5
• Protein Family: MAP kinase-activated protein kinase
• UniProt Gene Name: MAPKAPK5
• UniProt Synonym Gene Names: PRAK; MAPK-activated protein kinase 5; MAPKAP kinase 5; MAPKAP-K5; MAPKAPK-5; MK-5; MK5; PRAK
• UniProt Entry Name: MAPK5_HUMAN

NCBI Description

The protein encoded by this gene is a tumor suppressor and member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. The encoded protein is found in the nucleus but translocates to the cytoplasm upon phosphorylation and activation. This kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2012]

Uniprot Description

MAPKAPK5: a member of the MAPKAPK family of protein kinases. Activated through phosphorylation by MAP kinases including ERK, p38-alpha, and MAPp38-beta In response to cellular stress and proinflammatory cytokines. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Tumor suppressor; EC 2.7.11.1; Protein kinase, CAMK; CAMK group; MAPKAPK family; MAPKAPK subfamily

Chromosomal Location of Human Ortholog: 12q24.13

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function: calcium-dependent protein serine/threonine kinase activity; calmodulin binding; calmodulin-dependent protein kinase activity; MAP kinase kinase activity; p53 binding; protein binding; protein serine/threonine kinase activity

Biological Process: negative regulation of TOR signaling pathway; peptidyl-serine phosphorylation; positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase; protein amino acid autophosphorylation; Ras protein signal transduction; regulation of translation; signal transduction