EEF1A1 blocking peptide

EEF1A1 Antibody (C-term) Blocking Peptide

• Gene Names: EEF1A1; CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; LENG7; eEF1A-1; GRAF-1EF; HNGC:16303
• Synonyms: EEF1A1; EEF1A1 Antibody (C-term) Blocking Peptide; Elongation factor 1-alpha 1; EF-1-alpha-1; Elongation factor Tu; EF-Tu; Eukaryotic elongation factor 1 A-1; eEF1A-1; Leukocyte receptor cluster member 7; EEF1A; EF1A; LENG7; EEF1A1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human EEF1A1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 462

• Cellular Location: Cytoplasm. Nucleus. Nucleus, nucleolus. Note: Colocalizes with DLC1 at actin-rich regions in the cell periphery. Translocates together with ZPR1 from the cytoplasm to the nucleus and nucleolus after treatment with mitogens
• Tissue Location: Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for EEF1A1 blocking peptide

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.

NCBI and Uniprot Product Information

• NCBI GI #: 55584035
• NCBI GeneID: 1915
• UniProt Accession #: P68104; P04719; P04720; Q6IQ15
• Molecular Weight: 47,869 Da
• NCBI Official Full Name: Elongation factor 1-alpha 1
• NCBI Official Synonym Full Names: eukaryotic translation elongation factor 1 alpha 1
• NCBI Official Symbol: EEF1A1
• NCBI Official Synonym Symbols: CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; LENG7; eEF1A-1; GRAF-1EF; HNGC:16303
• NCBI Protein Information: elongation factor 1-alpha 1
• UniProt Protein Name: Elongation factor 1-alpha 1
• UniProt Gene Name: EEF1A1
• UniProt Synonym Gene Names: EEF1A; EF1A; LENG7; EF-1-alpha-1; EF-Tu; eEF1A-1
• UniProt Entry Name: EF1A1_HUMAN

NCBI Description

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008]

Uniprot Description

EEF1A1: an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. Promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Protein type: Nucleolus; Translation elongation; Translation

Chromosomal Location of Human Ortholog: 6q14.1

Cellular Component: cortical actin cytoskeleton; cytoplasm; cytosol; eukaryotic translation elongation factor 1 complex; extracellular space; membrane; nucleolus; nucleus

Molecular Function: GTP binding; GTPase activity; protein binding; protein kinase binding; translation elongation factor activity; tRNA binding

Biological Process: translational elongation