ACE2 (SARS Receptor) Blocking Peptide | ACE2 blocking peptide
ACE2 (SARS Receptor) Antibody (C-term) Blocking Peptide
Background:
ACE2 cDNA encodes a deduced 805-amino acid protein containing a potential 17-amino acid N-Terminal signal peptide and a putative 22-amino acid C-Terminal membrane anchor. It also possesses a zinc metalloprotease consensus sequence and a conserved glutamine residue that may function as a third zinc ligand. ACE2 is expressed predominantly in vascular endothelial cells of the heart and kidney. ACE converts angiotensin l to angiotensin ll, ACE2 converts angiotensin l to angiotensin 1-9, which has 9 amino acids. Angiotensin ll is a potent blood vessel constrictor, while angiotensin 1-9 does not impact blood vessels but is cleaved by ACE to a shorter peptide, angiotensin 1-7, which is a blood vessel dilator. Spike proteins of coronaviruses, including the SARS coronavirus. Bind with cellular receptors to mediate infection of target cells. ACE2 binds the S1 domain of the SARS coronavirus S protein. SARS coronavirus replicates efficiently on ACE2-Transfected but not mock-transfected 293T cells. Anti-ACE2 but not anti-ACE1 antibody blocks viral replication on Vero E6 cells. It has been proposed the ACE2 is a functional receptor of SARS coronavirus.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]
Uniprot Description
ACE2: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin- 13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. Interacts with ITGB1. Interacts with SARS-CoV and HCoV- NL63 spike glycoprotein. Up-regulated in failing heart. Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells. Expressed in heart, kidney, testis, and gastrointestinal system. Activated by chloride and fluoride, but not bromide. Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. Belongs to the peptidase M2 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; EC 3.4.17.23; Protease
Chromosomal Location of Human Ortholog: Xp22
Cellular Component: cell surface; extracellular region; extracellular space; lipid raft; plasma membrane
Molecular Function: carboxypeptidase activity; endopeptidase activity; glycoprotein binding; metallocarboxypeptidase activity; metallopeptidase activity; peptidyl-dipeptidase activity; protein binding; viral receptor activity; zinc ion binding
Biological Process: angiotensin catabolic process in blood; angiotensin maturation; angiotensin mediated drinking behavior; entry of virus into host cell; receptor biosynthetic process; regulation of cell proliferation; regulation of cytokine production; regulation of inflammatory response; regulation of systemic arterial blood pressure by renin-angiotensin; regulation of vasoconstriction; regulation of vasodilation; virion attachment, binding of host cell surface receptor
Research Articles on ACE2
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Product Notes
The ACE2 ace2 (Catalog #AAA9225887) is a Blocking Peptide and is intended for research purposes only. The product is available for immediate purchase. General recommendations for using blocking peptides in western blot and immunostaining techniques are described below. The precise conditions should be optimized for a particular assay. Protocol: Peptides are used to block antibody binding to its target. In order to visualize the inhibitory effect of the peptide, they are usually used at 10X to 100X excess compared to antibody molarity. We recommend using a 1X, 10X and 100X excess of peptide first, and then to narrow this range if a more accurate study is required. Calculation for antibodies manufactured at 0.25 mg/ml: Using the antibody at 1:100 dilution as recommended corresponds to 2.5 ug/ml. Estimating the MW of an antibody at 150,000 Da, the final antibody concentration is ca. 17 nM. A peptide of 15 residues long has an average MW of 1650 Da (110 Da multiplied by 15). For an excess of 100X of peptide over the antibody used at 17 nM, a concentration of 1.7 uM or 1.7 uM × 1650 = 2.8 ug/ml, is needed. Since one antibody binds two peptides, 5.6 ug/ml is 100X excess. Important Note: It is very important to mix the antibody with the peptide before incubation with the cell lysate or onto slide. Otherwise, you may not be able to disrupt antibody binding from native target. Researchers should empirically determine the suitability of the ACE2 ace2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACE2 (SARS Receptor), Blocking Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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