MAP4K4 (S801) Blocking Peptide | MAP4K4 blocking peptide

Phospho-MAP4K4(S801) Antibody Blocking peptide

• Gene Names: MAP4K4; HGK; NIK; MEKKK4; FLH21957; HEL-S-31
• Synonyms: MAP4K4 (S801); Phospho-MAP4K4(S801) Antibody Blocking peptide; Mitogen-activated protein kinase kinase kinase kinase 4; HPK/GCK-like kinase HGK; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; Nck-interacting kinase; MAP4K4; HGK; KIAA0687; NIK; MAP4K4 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the region of human Phospho-MAP4K4-S801. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 1239

• Cellular Location: Cytoplasm.
• Tissue Location: Appears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for MAP4K4 blocking peptide

Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. Phosphorylates SMAD1 on Thr-322.

NCBI and Uniprot Product Information

• NCBI GI #: 29427585
• NCBI GeneID: 9448
• UniProt Accession #: O95819; O75172; Q9NST7
• Molecular Weight: 141,929 Da
• NCBI Official Full Name: Mitogen-activated protein kinase kinase kinase kinase 4
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase kinase 4
• NCBI Official Symbol: MAP4K4
• NCBI Official Synonym Symbols: HGK; NIK; MEKKK4; FLH21957; HEL-S-31
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase kinase 4
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase kinase 4
• Protein Family: Mitogen-activated protein kinase kinase kinase kinase
• UniProt Gene Name: MAP4K4
• UniProt Synonym Gene Names: HGK; KIAA0687; NIK; MEK kinase kinase 4; MEKKK 4
• UniProt Entry Name: M4K4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

HGK: a Ser/Thr kinase of the STE20 subfamily. May play a role in the response to environmental stress and cytokines such as TNF-alpha. May interact with the SH3 domain of the adapter proteins Nck. Binds, via its CNH regulatory domain, to the N- terminal region of SPG3A. Upregulated in primary tumors and tumor cell lines. Required for anchorage-independent growth and ras-dependent focus formation in multiple cell lines. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; STE group; STE20 family; MSN subfamily

Chromosomal Location of Human Ortholog: 2q11.2-q12

Cellular Component: cytoplasm

Molecular Function: ATP binding; creatine kinase activity; protein binding; protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: microvillus biogenesis; negative regulation of apoptosis; protein amino acid phosphorylation; regulation of JNK cascade