RNF128 blocking peptide

RNF128 Antibody (Center) Blocking Peptide

• Gene Names: RNF128; GRAIL
• Synonyms: RNF128; RNF128 Antibody (Center) Blocking Peptide; E3 ubiquitin-protein ligase RNF128; 632-; Gene related to anergy in lymphocytes protein; GRAIL; RING finger protein 128; RNF128 blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 428

• Cellular Location: Endomembrane system; Single- pass membrane protein. Cytoplasm, cytoskeleton Cytoplasm, perinuclear region. Note: Localized in an asymmetric perinuclear punctate manner. Localizes to the internal pool of the transferrin recycling endosomal pathway. Partially colocalized with the endoplasmic reticulum resident HSPA5, with Golgi resident STX5, and with the late endosomal GTPase RAB7A (By similarity).
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for RNF128 blocking peptide

E3 ubiquitin-protein ligase that catalyzes 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains formation. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription, thereby playing an important role in the induction of the anergic phenotype, a long-term stable state of T-lymphocyte unresponsiveness to antigenic stimulation associated with the blockade of interleukin production. Ubiquitinates ARPC5 with 'Lys- 48' linkages and COR1A with 'Lys-63' linkages leading to their degradation, down-regulation of these cytosleletal components results in impaired lamellipodium formation and reduced accumulation of F-actin at the immunological synapse. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals.

NCBI and Uniprot Product Information

• NCBI GI #: 74751443
• NCBI GeneID: 79589
• UniProt Accession #: Q8TEB7; Q6PH80; Q6ZTJ8; Q96RF3; Q9H5E4; A0PJI4
• Molecular Weight: 44,617 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF128
• NCBI Official Synonym Full Names: ring finger protein 128, E3 ubiquitin protein ligase
• NCBI Official Symbol: RNF128
• NCBI Official Synonym Symbols: GRAIL
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF128
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF128
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF128
• UniProt Synonym Gene Names: GRAIL
• UniProt Entry Name: RN128_HUMAN

NCBI Description

The protein encoded by this gene is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of this gene in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of this gene was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

RNF128: E3 ubiquitin-protein ligase that catalyzes polyubiquitin chains. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription and this activity is likely to be mediated by E3 ligase activity. Plays an important role in the induction of the anergic phenotype. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural- inducing signals. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.19; EC 6.3.2.-; Ligase; Membrane protein, integral; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: Xq22.3

Molecular Function: protein binding

Biological Process: regulation of protein stability