APG4C Blocking Peptide | ATG4C blocking peptide

APG4C Antibody (Center) Blocking Peptide

• Gene Names: ATG4C; APG4C; AUTL1; AUTL3; APG4-C
• Synonyms: APG4C; APG4C Antibody (Center) Blocking Peptide; Cysteine protease ATG4C; 3422-; AUT-like 3 cysteine endopeptidase; Autophagin-3; Autophagy-related cysteine endopeptidase 3; Autophagy-related protein 4 homolog C; ATG4C; AUTL1; AUTL3; ATG4C blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the Center region of human Autophagy APG4C. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 458

• Cellular Location: Cytoplasm.
• Tissue Location: Highly expressed in skeletal muscle, heart, liver and testis.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ATG4C blocking peptide

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation (By similarity). Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.

NCBI and Uniprot Product Information

• NCBI GI #: 61211867
• NCBI GeneID: 84938
• UniProt Accession #: Q96DT6; Q96K04; A6NLR8; D3DQ58
• Molecular Weight: 52,497 Da
• NCBI Official Full Name: Cysteine protease ATG4C
• NCBI Official Synonym Full Names: autophagy related 4C cysteine peptidase
• NCBI Official Symbol: ATG4C
• NCBI Official Synonym Symbols: APG4C; AUTL1; AUTL3; APG4-C
• NCBI Protein Information: cysteine protease ATG4C
• UniProt Protein Name: Cysteine protease ATG4C
• Protein Family: Cysteine protease
• UniProt Gene Name: ATG4C
• UniProt Synonym Gene Names: APG4C; AUTL1; AUTL3
• UniProt Entry Name: ATG4C_HUMAN

NCBI Description

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

ATG4C: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Highly expressed in skeletal muscle, heart, liver and testis. Inhibited by N-ethylmaleimide. Belongs to the peptidase C54 family.

Protein type: Protease; Autophagy; EC 3.4.22.-; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1p31.3

Cellular Component: cytoplasm; cytosol; extracellular region

Molecular Function: cysteine-type endopeptidase activity; peptidase activity

Biological Process: autophagic vacuole formation; autophagy; C-terminal protein lipidation; mitochondrion degradation; protein delipidation; protein processing; protein targeting to membrane; proteolysis