Rabbit anti-Human, Mouse ATG4C Polyclonal Antibody | anti-ATG4C antibody

ATG4C Antibody

Cat. Num. AAA9405139

• Gene Names: ATG4C; APG4C; AUTL1; AUTL3; APG4-C
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: ATG4C; Polyclonal Antibody; ATG4C Antibody; APG4 autophagy 4 homolog C; APG4-C; APG4C; AUT-like 3 cysteine endopeptidase; AUTL1; anti-ATG4C antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total ATG4C protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 458

• Applicable Applications for anti-ATG4C antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500~1:3000; Immunohistochemistry: 1:50~1:100; Immunofluorescence: 1:100~1:500
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from internal of human ATG4C.
• Target Name: ATG4C
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from K562 cells and COS7 cells, using ATG4C antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using ATG4C antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HuvEc cells, using ATG4C antibody.)

Related Product Information for anti-ATG4C antibody

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation By similarity. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.Marino G., J. Biol. Chem. 278:3671-3678(2003).Chen J.M., Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.Ota T., Nat. Genet. 36:40-45(2004).

Product Categories/Family for anti-ATG4C antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 30410844
• NCBI GeneID: 84938
• NCBI Accession #: NP_116241.2
• NCBI GenBank Nucleotide #: NM_032852.3
• UniProt Accession #: Q96DT6; Q96K04; A6NLR8; D3DQ58
• Molecular Weight: 52,497 Da
• NCBI Official Full Name: cysteine protease ATG4C
• NCBI Official Synonym Full Names: autophagy related 4C, cysteine peptidase
• NCBI Official Symbol: ATG4C
• NCBI Official Synonym Symbols: APG4C; AUTL1; AUTL3; APG4-C
• NCBI Protein Information: cysteine protease ATG4C
• UniProt Protein Name: Cysteine protease ATG4C
• Protein Family: Cysteine protease
• UniProt Gene Name: ATG4C
• UniProt Synonym Gene Names: APG4C; AUTL1; AUTL3
• UniProt Entry Name: ATG4C_HUMAN

NCBI Description

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

ATG4C: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Highly expressed in skeletal muscle, heart, liver and testis. Inhibited by N-ethylmaleimide. Belongs to the peptidase C54 family.

Protein type: EC 3.4.22.-; Protease; Ubiquitin conjugating system; Autophagy

Chromosomal Location of Human Ortholog: 1p31.3

Cellular Component: cytoplasm; extracellular region; cytosol

Molecular Function: peptidase activity; cysteine-type endopeptidase activity

Biological Process: protein delipidation; mitochondrion degradation; autophagy; C-terminal protein lipidation; protein processing; proteolysis; protein targeting to membrane; autophagic vacuole formation; cellular response to nitrogen starvation