APG4A Blocking Peptide | ATG4A blocking peptide

APG4A Antibody (C-term) Blocking Peptide

• Gene Names: ATG4A; APG4A; AUTL2
• Synonyms: APG4A; APG4A Antibody (C-term) Blocking Peptide; Cysteine protease ATG4A; 3422-; AUT-like 2 cysteine endopeptidase; Autophagin-2; Autophagy-related cysteine endopeptidase 2; Autophagy-related protein 4 homolog A; hAPG4A; ATG4A; AUTL2; ATG4A blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human Autophagy APG4A. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 398

• Cellular Location: Cytoplasm.
• Tissue Location: Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ATG4A blocking peptide

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.

NCBI and Uniprot Product Information

• NCBI GI #: 61211859
• NCBI GeneID: 115201
• UniProt Accession #: Q8WYN0; O95534; Q5JYY9; Q5JYZ0; Q86VE5; Q96KQ0; Q96KQ1; A6NCH2; B2RAZ7; D3DUY0
• Molecular Weight: 42,453 Da
• NCBI Official Full Name: Cysteine protease ATG4A
• NCBI Official Synonym Full Names: autophagy related 4A cysteine peptidase
• NCBI Official Symbol: ATG4A
• NCBI Official Synonym Symbols: APG4A; AUTL2
• NCBI Protein Information: cysteine protease ATG4A
• UniProt Protein Name: Cysteine protease ATG4A
• Protein Family: Cysteine protease
• UniProt Gene Name: ATG4A
• UniProt Synonym Gene Names: APG4A; AUTL2; hAPG4A
• UniProt Entry Name: ATG4A_HUMAN

NCBI Description

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. [provided by RefSeq, Mar 2016]

Uniprot Description

ATG4A: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver. Inhibited by N-ethylmaleimide. Belongs to the peptidase C54 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Microtubule-binding; Protease; EC 3.4.22.-

Chromosomal Location of Human Ortholog: Xq22.3

Cellular Component: cytoplasm; cytosol

Molecular Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity; protein binding

Biological Process: autophagic vacuole formation; C-terminal protein lipidation; mitochondrion degradation; protein delipidation; protein processing; protein targeting to membrane; proteolysis