Highly validated and characterized monoclonal/polyclonal
antibodies and recombinant
proteins
The majority of AAA Biotech’s antibodies are highly validated and can be use in multiple
applications such as ELISA, FC,
ICC, IF, IHC, IP, WB, etc. We have antibodies available for rare species, in multiple conjugated
forms or recombinant
antibodies.
As for our high quality proteins, the majority have 90% purity, detected by SDS-PAGE while some are
available in
different tags such as Flag, GST, His, MBP, etc. We also carry high quality native and biologically
active proteins.
AAA Biotech is constantly working to expand our capacity to provide recombinant proteins and
antibodies to most
target proteins.
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Query
Database
1.75 ms
select p.*, pd.*,
ifnull(pdns.ncbi_summary, 'N/A') as ncbi_summary_pdns,
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ifnull(pe.products_description_extra, 'N/A') as products_description_extra
from products p
LEFT OUTER JOIN products_description pd on p.products_id = pd.products_id
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LEFT OUTER JOIN products_extra pe on p.products_id = pe.products_id
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Query
Database
1.38 ms
SELECT `options_values_price` as `price`, `products_options_values_name` as `package`
FROM `products_attributes`
JOIN `products_options_values` ON `products_options_values`.`products_options_values_id` = `products_attributes`.`options_values_id`
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Database (4 total Queries, 4 of them unique across 2 Connections)
Time
Query String
1.88 ms
SELECT `p`.*, `pd`.*, IFNULL(pdns.ncbi_summary, "N/A") as ncbi_summary_pdns, IFNULL(pdns.sp_comments, "N/A") as sp_comments_pdns, IFNULL(pdns.ncbi_research_articles, "N/A") as ncbi_research_articles_pdns, IFNULL(pe.products_description_extra, "N/A") as products_description_extra
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ifnull(pe.products_description_extra, 'N/A') as products_description_extra
from products p
LEFT OUTER JOIN products_description pd on p.products_id = pd.products_id
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LEFT OUTER JOIN products_extra pe on p.products_id = pe.products_id
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This assay has high sensitivity and excellent specificity for detection of LTB4. No significant cross-reactivity or interference between LTB4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LTB4 and all the analogues, therefore, cross reaction may still exist in some cases.
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Principle of the Assay: LTB4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-LTB4 antibody and an LTB4-HRP conjugate. The assay sample and buffer are incubated together with LTB4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LTB4 concentration since LTB4 from samples and LTB4-HRP conjugate compete for the anti-LTB4 antibody binding site. Since the number of sites is limited, as more sites are occupied by LTB4 from the sample, fewer sites are left to bind LTB4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LTB4 concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This LTB4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human LTB4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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title
Human Leukotriene B4 ELISA Kit | LTB4 elisa kit
datasheet
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Available methods (45)
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This assay has high sensitivity and excellent specificity for detection of LTB4. No significant cross-reactivity or interference between LTB4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LTB4 and all the analogues, therefore, cross reaction may still exist in some cases.
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Principle of the Assay: LTB4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-LTB4 antibody and an LTB4-HRP conjugate. The assay sample and buffer are incubated together with LTB4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LTB4 concentration since LTB4 from samples and LTB4-HRP conjugate compete for the anti-LTB4 antibody binding site. Since the number of sites is limited, as more sites are occupied by LTB4 from the sample, fewer sites are left to bind LTB4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LTB4 concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This LTB4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human LTB4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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This assay has high sensitivity and excellent specificity for detection of LTB4. No significant cross-reactivity or interference between LTB4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LTB4 and all the analogues, therefore, cross reaction may still exist in some cases.
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Principle of the Assay: LTB4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-LTB4 antibody and an LTB4-HRP conjugate. The assay sample and buffer are incubated together with LTB4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LTB4 concentration since LTB4 from samples and LTB4-HRP conjugate compete for the anti-LTB4 antibody binding site. Since the number of sites is limited, as more sites are occupied by LTB4 from the sample, fewer sites are left to bind LTB4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LTB4 concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This LTB4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human LTB4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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$value[0]['_source']['products_description']
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Bovine C3a monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
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aaa22712 bovine no cross reaction with other factors typical testing data standard curve for reference only aaa22712_sc elisa kit complement 3a c3a anaphylatoxin chemotactic receptor 1 c3ar1 az3b c3ar hnfag09 53,864 da c3r1 r c3ar_human 4757888 np_004045.1 q16581 nm_004054.3 o43771 q92868 u28488 mrna samples serum plasma or cell culture supernatant assay type quantitative sandwich detection range 20 ng ml 0.312 sensitivity up to 0.06 intra precision receptor1 range20
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Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Mouse C3a monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
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aaa13039 mouse typical testing data standard curve for reference only aaa13039_sc elisa kit complement 3a c3a anaphylatoxin chemotactic receptor component 1 c3ar1 az3b c3ar hnfag09 r 3 53,864 da c3r1 c3ar_human 4757888 np_004045.1 q16581 nm_004054.2 o43771 q92868 605246 samples serum plasma or cell culture supernatant assay type quantitative sandwich detection range 20 ng ml 0.312 sensitivity up to 0.06 intra precision component1 r3 range20
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Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine C3a monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
⇄⧉search_terms => string (487) "aaa12488 porcine no cross reaction with other factors typical testing data s...
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aaa12488 porcine no cross reaction with other factors typical testing data standard curve for reference only aaa12488_sc elisa kit complement 3a c3a anaphylatoxin chemotactic receptor component 1 c3ar1 az3b c3ar hnfag09 r 3 53,864 da c3r1 c3ar_human 4757888 np_004045.1 q16581 nm_004054.2 o43771 q92868 605246 samples serum plasma or cell culture supernatant assay type quantitative sandwich detection range 50 ng ml 0.78 sensitivity up to 0.1 intra precision component1 r3 range50 to0.1
⇄specificity => string (74) "Specifically recognize C5a, no obvious cross reaction with other analogues"
$value[3]['_source']['specificity']
⇄purity => string (3) "N/A"
$value[3]['_source']['purity']
⇄form => string (3) "N/A"
$value[3]['_source']['form']
⇄concentration => string (3) "N/A"
$value[3]['_source']['concentration']
⇄⧉storage_stability => string (156) "Store entire kit at 2-8C for short-term. For longer-term, please store the m...
$value[3]['_source']['storage_stability']
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Assay Type||Sandwich!!Samples||Serum, plasma, cell culture supernatant and other biological samples!!Detection Range||0.156-10ng/ml!!Sensitivity||0.094ng/ml
⇄⧉etc_term2 => string (270) "Intra-assay Precision||Intra-assay Precision: samples with low, medium and h...
$value[3]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.!!Inter-assay Precision||Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
⇄⧉products_description => string (2022) "Background: C5a is a small peptide molecule in the complement system, formed...
$value[3]['_source']['products_description']
Background: C5a is a small peptide molecule in the complement system, formed by the cleavage of complement C5, and has strong chemotactic activity. It is a critical regulatory molecule in inflammation and immune responses, capable of attracting various immune cells (such as neutrophils, monocytes, T cells, and macrophages) to the site of inflammation, and participating in the activation, aggregation, and mediation of immune cells and inflammatory responses. C5a promotes the chemotaxis and activation of immune cells by interacting with its specific receptor C5aR, inducing the activation of intracellular signaling pathways, ultimately resulting in various biological effects such as extracellular matrix degradation, intracellular signal transduction, cell apoptosis, and production of inflammatory mediators. In addition to its role in normal immune responses, excessive production and abnormal activation of C5a are also associated with various diseases such as autoimmune diseases, infectious diseases, cardiovascular diseases, neurological diseases, and cancer.<br><br>Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti C5a antibody was pre-coated onto the 96-well plate. The biotin conjugated anti C5a antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with C5a conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of C5a in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
⇄products_references => string (3) "N/A"
$value[3]['_source']['products_references']
⇄⧉products_related_diseases => string (204) "Inflammation||60!!Immune System Diseases||27!!Cardiovascular Diseases||15!!N...
$value[3]['_source']['products_related_diseases']
Inflammation||60!!Immune System Diseases||27!!Cardiovascular Diseases||15!!Necrosis||13!!Kidney Diseases||10!!Nervous System Diseases||10!!Lung Diseases||8!!Atherosclerosis||7!!Fibrosis||5!!Lung Injury||4
⇄⧉search_terms => string (545) "aaa17637 monkey this assay has high sensitivity and excellent specificity fo...
$value[3]['_source']['search_terms']
aaa17637 monkey this assay has high sensitivity and excellent specificity for detection of c5a no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa17637_sc elisa kit complement component 5a receptor 1 c5ar1 c5ar c5r1 cd88 anaphylatoxin chemotactic 39,336 da r cd_antigen c5ar1_human 29169287 aao65969.1 p21730 113995 samples serum plasma tissue homogenates other biological fluids type quantitative sandwich range 0.156 10ng ml 0.094ng intra precision cv receptor1
⇄⧉products_description => string (825) "Principle of the Assay: As mentioned above, this kit utilizes the Double Ant...
$value[4]['_source']['products_description']
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Rabbit C3 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
⇄⧉search_terms => string (456) "aaa13185 rabbit typical testing data standard curve for reference only aaa13...
$value[4]['_source']['search_terms']
aaa13185 rabbit typical testing data standard curve for reference only aaa13185_sc elisa kit complement 3a c3a anaphylatoxin chemotactic receptor component 1 c3ar1 az3b c3ar hnfag09 r 3 53,864 da c3r1 c3ar_human 4757888 np_004045.1 q16581 nm_004054.2 o43771 q92868 605246 samples serum plasma or cell culture supernatant assay type quantitative sandwich detection range 300 ng ml 4.7 sensitivity up to 0.8 intra precision component1 r3 range300 ml4.7 to0.8
⇄⧉products_description => string (823) "Principle of the Assay: As mentioned above, this kit utilizes the Double Ant...
$value[5]['_source']['products_description']
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Rat C3a monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
⇄⧉search_terms => string (538) "aaa12467 rat no cross reaction with other factors typical testing data stand...
$value[5]['_source']['search_terms']
aaa12467 rat no cross reaction with other factors typical testing data standard curve for reference only aaa12467_sc elisa kit complement 3a c3a anaphylatoxin chemotactic receptor component 1 c3ar1 az3b c3ar hnfag09 r 3 53,864 da c3r1 c3ar_human 4757888 np_004045.1 q16581 nm_004054.2 o43771 q92868 605246 samples serum plasma or cell culture supernatant and organizations in the natural recombinant c1q concentration assay type sandwich detection range 100 ng ml 1.56 sensitivity 0.5 intra precision component1 r3 range100 sensitivity0.5
⇄⧉storage_stability => string (503) "<b>Storage:</b> Avoid repeated freeze/thaw cycles.<br>Store at 2-8 degree C ...
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<b>Storage:</b> Avoid repeated freeze/thaw cycles.<br>Store at 2-8 degree C for one month.<br>Aliquot and store at -80 degree C for 12 months. .<br><b>Stability Test:</b> The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
⇄app_tested => string (56) "Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)"
$value[6]['_source']['app_tested']
⇄app_notes => string (75) "(May be suitable for use in other assays to be determined by the end user.)"
Predicted Molecular Mass: 38.3kDa<br>Accurate Molecular Mass: 38kDa as determined by SDS-PAGE reducing conditions.
⇄⧉ncbi_pathways => string (507) "Activation Of C3 And C5 Pathway||926093!!Adaptive Immune System Pathway||926...
$value[6]['_source']['ncbi_pathways']
Activation Of C3 And C5 Pathway||926093!!Adaptive Immune System Pathway||926013!!Alternative Complement Activation Pathway||926092!!Chagas Disease (American Trypanosomiasis) Pathway||147810!!Chagas Disease (American Trypanosomiasis) Pathway||147795!!Class A/1 (Rhodopsin-like Receptors) Pathway||926991!!Complement Activation, Classical Pathway||198379!!Complement And Coagulation Cascades Pathway||198335!!Complement And Coagulation Cascades Pathway||83270!!Complement And Coagulation Cascades Pathway||484
⇄sp_protein_name => string (13) "Complement C3"
$value[6]['_source']['sp_protein_name']
⇄⧉sp_protein_name_syn => string (177) "HSE-MSFCleaved into the following 11 chains:Complement C3 beta chainCompleme...
$value[6]['_source']['sp_protein_name_syn']
HSE-MSFCleaved into the following 11 chains:Complement C3 beta chainComplement C3 alpha chainC3a anaphylatoxinAcylation stimulating protein; ASP; Alternative name(s):; C3adesArg
aaa20262 e.coli > 90 20mm tris 150mm nacl ph8.0 containing 1mm edta dtt 0.01 skl 5 trehalose and proclin 300 positive control immunogen sds page western blot wb may be suitable for use in other assays to determined by the end user sequence information aaa20262_seq testing data aaa20262_td2 aaa20262_sds3 recombinant protein complement component 3a c3a c3 3 asp plp hse msf ai255234 factor 3d acylation stimulating predicted molecular mass 38.3kda accurate 38kda as reducing conditions msfcleaved into following 11 chains:complement beta chaincomplement alpha chainc3a anaphylatoxinacylation alternative name s c3adesarg co3_mouse 126518317 np_033908.2 p01027 nm_009778.2 q61370 q80xp1 organism species mus musculus mouse source prokaryotic expression residues ser671~arg748 tags n terminal his gst tag subcellular location secreted traits freeze dried powder isoelectric point 8.4 usage reconstitute a concentration of 0.1 1.0 mg ml do not
vortex >90 skl5 proclin300 c33 following11 point8.4 of0.1
⇄⧉products_description => string (823) "Principle of the Assay: As mentioned above, this kit utilizes the Double Ant...
$value[7]['_source']['products_description']
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Rat C4b monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
⇄products_references => string (3) "N/A"
$value[7]['_source']['products_references']
⇄⧉products_related_diseases => string (216) "Inflammation||171!!Nervous System Diseases||142!!Brain Diseases||111!!Alzhei...
$value[7]['_source']['products_related_diseases']
Inflammation||171!!Nervous System Diseases||142!!Brain Diseases||111!!Alzheimer Disease||89!!Kidney Diseases||81!!Necrosis||71!!Respiratory Tract Diseases||70!!Malaria||62!!Malaria, Falciparum||53!!Liver Diseases||49
⇄products_categories => string (3) "N/A"
$value[7]['_source']['products_categories']
⇄ncbi_full_name => string (36) "complement receptor type 1 isoform S"
⇄⧉specificity => string (370) "This assay has high sensitivity and excellent specificity for detection of C...
$value[8]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of C5. No significant cross-reactivity or interference between C5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between C5 and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value[8]['_source']['purity']
⇄form => string (3) "N/A"
$value[8]['_source']['form']
⇄concentration => string (3) "N/A"
$value[8]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
Assay Type||Quantitative Sandwich!!Samples||Serum, plasma, cell culture supernatants, body fluid and tissue homogenate!!Sensitivity||1.0 ng/mL
⇄⧉etc_term2 => string (194) "Intended Uses||This C5 ELISA kit is a 1.5 hour solid-phase ELISA designed fo...
$value[8]['_source']['etc_term2']
Intended Uses||This C5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey C5. This ELISA kit for research use only, not for therapeutic applications!
⇄⧉products_description => string (1484) "Principle of the Assay: C5 ELISA kit applies the quantitative sandwich enzym...
$value[8]['_source']['products_description']
Principle of the Assay: C5 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for C5. Standards or samples are then added to the microtiter plate wells and C5 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of C5 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for C5 are added to each well to "sandwich" the C5 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain C5 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C5 concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This C5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey C5. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄products_references => string (3) "N/A"
$value[8]['_source']['products_references']
⇄⧉products_related_diseases => string (234) "Nervous System Diseases||2178!!Inflammation||695!!Cardiovascular Diseases||6...
$value[8]['_source']['products_related_diseases']
Nervous System Diseases||2178!!Inflammation||695!!Cardiovascular Diseases||635!!Vascular Diseases||619!!Complement component 5 deficiency||593!!Autoimmune Diseases||457!!Hemolysis||446!!Kidney Diseases||407!!Necrosis||219!!Anemia||212
complement C5; prepro-C5; C5a anaphylatoxin; anaphylatoxin C5a analog; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
⇄ncbi_chrom_loc => string (3) "N/A"
$value[8]['_source']['ncbi_chrom_loc']
⇄ncbi_gene_id => string (3) "727"
$value[8]['_source']['ncbi_gene_id']
⇄ncbi_mol_weight => string (10) "188,305 Da"
$value[8]['_source']['ncbi_mol_weight']
⇄⧉ncbi_pathways => string (450) "Activation Of C3 And C5 Pathway||106412!!Class A/1 (Rhodopsin-like Receptors...
$value[8]['_source']['ncbi_pathways']
Activation Of C3 And C5 Pathway||106412!!Class A/1 (Rhodopsin-like Receptors) Pathway||106357!!Complement Activation, Classical Pathway||198823!!Complement And Coagulation Cascades Pathway||83073!!Complement And Coagulation Cascades Pathway||484!!Complement Cascade Pathway||106405!!G Alpha (i) Signalling Events Pathway||119550!!GPCR Downstream Signaling Pathway||119548!!GPCR Ligand Binding Pathway||161020!!Herpes Simplex Infection Pathway||377873
⇄sp_protein_name => string (13) "Complement C5"
$value[8]['_source']['sp_protein_name']
⇄⧉sp_protein_name_syn => string (199) "C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4Cleaved int...
$value[8]['_source']['sp_protein_name_syn']
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4Cleaved into the following 4 chains:Complement C5 beta chain; Complement C5 alpha chain; C5a anaphylatoxin; Complement C5 alpha' chain
⇄⧉search_terms => string (692) "aaa16957 monkey typical testing data standard curve for reference only aaa16...
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aaa16957 monkey typical testing data standard curve for reference only aaa16957_td elisa kit complement component 5 c5 c5d c5a c5b eclzb cpamd4 prepro anaphylatoxin analog c3 and pzp like alpha 2 macroglobulin domain containing protein 4 188,305 da 4cleaved into the following chains:complement beta chain alpha' co5_human 109731812 aai13739.1 p01031 q14cj0 q27i61 615749 immunology samples serum plasma cell culture supernatants body fluid tissue homogenate assay type sandwich detection range 2.5 50ng ml sensitivity 1.0ng intended uses this is a 1.5 hour solid phase designed quantitative determination of research use not therapeutic applications! component5 alpha2 protein4 range2.5 a1.5
⇄⧉etc_term1 => string (156) "Samples||Serum, plasma, Cell Culture Supernatants, body fluid and tissue hom...
$value[9]['_source']['etc_term1']
Samples||Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate!!Assay Type||Sandwich!!Detection Range||1.0-25mg/L!!Sensitivity||0.1mg/L
⇄⧉etc_term2 => string (194) "Intended Uses||This C3 ELISA kit is a 1.5 hour solid-phase ELISA designed fo...
$value[9]['_source']['etc_term2']
Intended Uses||This C3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey C3. This ELISA kit for research use only, not for therapeutic applications!
⇄⧉products_description => string (1155) "<b>Principle of the assay: </b>C3 ELISA kit applies the competitive enzyme i...
$value[9]['_source']['products_description']
<b>Principle of the assay: </b>C3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-C3 antibody and an C3-HRP conjugate. The assay sample and buffer are incubated together with C3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C3 concentration since C3 from samples and C3-HRP conjugate compete for the anti-C3 antibody binding site. Since the number of sites is limited, as more sites are occupied by C3 from the sample, fewer sites are left to bind C3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C3 concentration in each sample is interpolated from this standard curve.
⇄⧉search_terms => string (807) "aaa16866 monkey typical testing data standard curve for reference only aaa16...
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aaa16866 monkey typical testing data standard curve for reference only aaa16866_td elisa kit complement component 3 c3 asp c3a c3b ahus5 armd9 cpamd1 hel s 62p prepro anaphylatoxin acylation stimulating protein cleavage product epididymis secretory sperm binding li and pzp like alpha 2 macroglobulin domain containing 1 187,148 da 1cleaved into the following 11 chains:complement beta chain aspalternative name c3adesarg co3_human 40786791 aar89906.1 p01024 a7e236 613779 immunology samples serum plasma cell culture supernatants body fluid tissue homogenate assay type sandwich detection range 1.0 25mg l sensitivity 0.1mg intended uses this is a 1.5 hour solid phase designed quantitative determination of research use not therapeutic applications! component3 alpha2 containing1 following11 range1.0 a1.5
⇄⧉specificity => string (385) "This assay has high sensitivity and excellent specificity for detection of C...
$value[10]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of C3alpha. No significant cross-reactivity or interference between C3alpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between C3alpha and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value[10]['_source']['purity']
⇄form => string (3) "N/A"
$value[10]['_source']['form']
⇄concentration => string (3) "N/A"
$value[10]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
Assay Type||Quantitative Competitive!!Samples||Serum, plasma, cell culture supernatants, body fluid and tissue homogenate!!Sensitivity||0.1 ng/mL
⇄etc_term2 => string (3) "N/A"
$value[10]['_source']['etc_term2']
⇄products_price => string (6) "0.0000"
$value[10]['_source']['products_price']
⇄products_weight => string (4) "5.00"
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⇄products_status => boolean true
$value[10]['_source']['products_status']
⇄products_tax_class_id => string (1) "1"
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⇄manufacturers_id => string (3) "720"
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$value[10]['_source']['products_ordered']
⇄language_id => string (1) "1"
$value[10]['_source']['language_id']
⇄products_name => string (22) "Complement Fragment 3a"
$value[10]['_source']['products_name']
⇄products_name_oem => string (39) "Canine Complement Fragment 3a ELISA Kit"
$value[10]['_source']['products_name_oem']
⇄products_name_syn => string (3) "N/A"
$value[10]['_source']['products_name_syn']
⇄products_gene_name => string (3) "C3a"
$value[10]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[10]['_source']['products_gene_name_syn']
⇄⧉products_description => string (1429) "Principle of the Assay: C3alpha ELISA kit applies the competitive enzyme imm...
$value[10]['_source']['products_description']
Principle of the Assay: C3alpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-C3alpha antibody and an C3alpha-HRP conjugate. The assay sample and buffer are incubated together with C3alpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C3alpha concentration since C3alpha from samples and C3alpha-HRP conjugate compete for the anti-C3alpha antibody binding site. Since the number of sites is limited, as more sites are occupied by C3alpha from the sample, fewer sites are left to bind C3alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C3alpha concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This C3alpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine C3alpha. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄⧉search_terms => string (962) "aaa17234 canine this assay has high sensitivity and excellent specificity fo...
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aaa17234 canine this assay has high sensitivity and excellent specificity for detection of c3alpha no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa17234_sc elisa kit complement fragment 3a c3a c3 component 3 asp c3b ahus5 armd9 cpamd1 hel s 62p prepro anaphylatoxin acylation stimulating protein cleavage product epididymis secretory sperm binding li pzp like alpha 2 macroglobulin domain containing 1 187,148 da 1cleaved into following 11 chains:complement beta chain aspalternative name c3adesarg co3_human 115298678 np_000055.2 p01024 nm_000064.2 a7e236 613779 immunology samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 0.1 ng ml component3 alpha2 containing1 following11 competitive0.1
⇄⧉specificity => string (175) "This assay has high sensitivity and excellent specificity for detection of r...
$value[11]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of rat C3a. No significant cross-reactivity or interference between rat C3a and analogues was observed.
⇄purity => string (3) "N/A"
$value[11]['_source']['purity']
⇄form => string (3) "N/A"
$value[11]['_source']['form']
⇄concentration => string (3) "N/A"
$value[11]['_source']['concentration']
⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
$value[11]['_source']['storage_stability']
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (327) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
$value[11]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
⇄products_price => string (6) "0.0000"
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⇄products_weight => string (4) "5.00"
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⇄products_status => boolean true
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⇄products_tax_class_id => string (1) "1"
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⇄manufacturers_id => string (3) "700"
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⇄products_ordered => string (1) "0"
$value[11]['_source']['products_ordered']
⇄language_id => string (1) "1"
$value[11]['_source']['language_id']
⇄products_name => string (27) "Complement fragment 3a, C3a"
⇄products_name_syn => string (70) "Rat Complement fragment 3a; C3a ELISA Kit; Complement fragment 3a; C3a"
$value[11]['_source']['products_name_syn']
⇄products_gene_name => string (3) "C3a"
$value[11]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[11]['_source']['products_gene_name_syn']
⇄⧉products_description => string (727) "Principle of the Assay||This assay employs the quantitative sandwich enzyme ...
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Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C3a has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C3a present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C3a is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C3a bound in the initial step. The color development is stopped and the intensity of the color is measured.
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aaa15306 rat this assay has high sensitivity and excellent specificity for detection of c3a no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa15306_td elisa kit complement fragment 3a samples serum plasma tissue homogenates type sandwich range 1.56 ng ml 100 0.39 intra precision within an cv <8 three known concentration were tested twenty times on one plate to assess inter assays <10 in ml100
⇄⧉specificity => string (181) "This assay has high sensitivity and excellent specificity for detection of r...
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This assay has high sensitivity and excellent specificity for detection of rabbit C3a. No significant cross-reactivity or interference between rabbit C3a and analogues was observed.
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Samples||Serum, plasma, tissue homogenates!!Assay Type||Quantitative Sandwich!!Detection Range||6.25 ng/ml -400 ng/ml!!Sensitivity||Typically less than 1.56 ng/ml.
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C3a has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C3a present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C3a is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C3a bound in the initial step. The color development is stopped and the intensity of the color is measured.
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aaa15419 rabbit this assay has high sensitivity and excellent specificity for detection of c3a no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa15419_td elisa kit complement fragment 3a samples serum plasma tissue homogenates type quantitative sandwich range 6.25 ng ml 400 typically less than 1.56 intra precision within an cv <8 three known concentration were tested twenty times on one plate to assess inter assays <10 in ml400
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This assay has high sensitivity and excellent specificity for detection of C4B. No significant cross-reactivity or interference between C4B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between C4B and all the analogues, therefore, cross reaction may still exist in some cases.
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Principle of the Assay: C4B ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-C4B antibody and an C4B-HRP conjugate. The assay sample and buffer are incubated together with C4B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C4B concentration since C4B from samples and C4B-HRP conjugate compete for the anti-C4B antibody binding site. Since the number of sites is limited, as more sites are occupied by C4B from the sample, fewer sites are left to bind C4B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C4B concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This C4B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat C4B. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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aaa16163 rat typical testing data standard curve for reference only aaa16163_td elisa kit complement component 4b c4b partial 1,094 da r9qa77_human 356601440 aet24773.1 immunology samples serum plasma cell culture supernatants body fluid and tissue homogenate assay type sandwich detection range 2.5 50ng ml sensitivity 1.0ng range2.5
⇄specificity => string (73) "Specifically recognize C7, no obvious cross reaction with other analogues"
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Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Assay Type||Sandwich!!Samples||Serum, plasma, cell culture supernatant and other biological samples!!Detection Range||1.563-100ng/ml!!Sensitivity||0.938ng/ml
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Intra-assay Precision||Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.!!Inter-assay Precision||Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
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Background: Complement component 7 (C7) is a 93-kDa serum glycoprotein encoded by the C7 gene. C7 interacts with other terminal complement components (C5b, C6, C8, and C9) to form a membrane attack complex (MAC), which functions as the cytolytic effector unit of the complement system. Diseases associated with C7 include Complement Component 7 Deficiency and Immunodeficiency Due To A Late Component Of Complement Deficiency.<br><br>Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti C7 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti C7 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with C7 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of C7 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
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aaa17727 human this assay has high sensitivity and excellent specificity for detection of c7 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa17727_sc elisa kit complement component 7 93,518 da co7_human 45580688 np_000578.2 p10643 nm_000587.2 q6p3t5 q92489 217070 samples serum plasma tissue homogenates other biological fluids type quantitative sandwich range 1.563 100ng ml 0.938ng intra precision cv component7
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This assay has high sensitivity and excellent specificity for detection of C3. No significant cross-reactivity or interference between C3 and analogues was observed.
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Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.
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aaa17333 rat this assay has high sensitivity and excellent specificity for detection of c3 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa17333_sc elisa kit complement component 3 asp c3a c3b ahus5 armd9 cpamd1 hel s 62p 187,148 da pzp like alpha 2 macroglobulin domain containing protein 1 c3bc co3_human 152012784 aai50180.1 p01024 a7e236 120700 samples serum plasma tissue homogenates other biological fluids type quantitative sandwich range 3.125 200ng ml 1.875ng intra precision cv component3 alpha2 protein1
⇄⧉specificity => string (179) "This assay has high sensitivity and excellent specificity for detection of m...
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This assay has high sensitivity and excellent specificity for detection of mouse C3a. No significant cross-reactivity or interference between mouse C3a and analogues was observed.
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C3a has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C3a present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C3a is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C3a bound in the initial step. The color development is stopped and the intensity of the color is measured.
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aaa15529 mouse this assay has high sensitivity and excellent specificity for detection of c3a no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa15529_td elisa kit complement fragment 3a samples serum plasma cell culture supernates tissue homogenates type quantitative sandwich range 0.156 ng ml 10 < 0.039 intra precision within an cv <8 three known concentration were tested twenty times on one plate to assess inter assays <10 in ml10
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This assay has high sensitivity and excellent specificity for detection of C3alpha. No significant cross-reactivity or interference between C3alpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between C3alpha and all the analogues, therefore, cross reaction may still exist in some cases.
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Samples||Serum, plasma, cell culture supernatants, body fluid and tissue homogenate!!Assay Type||Quantitative Competitive!!Sensitivity||1.0 ng/mL
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Intended Uses: This C3alpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse C3alpha. This ELISA kit for research use only, not for therapeutic or test applications!<br><br>Principle of the Assay: C3alpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-C3alpha antibody and an C3alpha-HRP conjugate. The assay sample and buffer are incubated together with C3alpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C3alpha concentration since C3alpha from samples and C3alpha-HRP conjugate compete for the anti-C3alpha antibody binding site. Since the number of sites is limited, as more sites are occupied by C3alpha from the sample, fewer sites are left to bind C3alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C3alpha concentration in each sample is interpolated from this standard curve.
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aaa17230 mouse this assay has high sensitivity and excellent specificity for detection of c3alpha no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa17230_sc elisa kit complement fragment 3a c3a c3 component 3 asp c3b ahus5 armd9 cpamd1 hel s 62p prepro anaphylatoxin acylation stimulating protein cleavage product epididymis secretory sperm binding li pzp like alpha 2 macroglobulin domain containing 1 187,148 da 1cleaved into following 11 chains:complement beta chain aspalternative name c3adesarg co3_human 115298678 np_000055.2 p01024 nm_000064.2 a7e236 613779 immunology samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 1.0 ng ml component3 alpha2 containing1 following11 competitive1.0
⇄⧉products_description => string (824) "Principle of the Assay: As mentioned above, this kit utilizes the Double Ant...
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Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Mouse C4 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
CD40/CD40L Signaling Pathway||138061!!Complement And Coagulation Cascades Pathway||83073!!Complement And Coagulation Cascades Pathway||484!!Complement Cascade Pathway||106405!!Immune System Pathway||106386!!Innate Immune System Pathway||106387!!Pertussis Pathway||218111!!Pertussis Pathway||218099!!Regulation Of Complement Cascade Pathway||576254
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aaa12570 mouse typical testing data standard curve for reference only aaa12570_sc elisa kit complement component 4 c4 binding protein alpha c4bpa prp c4bp c4b chain proline rich 67,033 da c4bpa_human 18490166 aah22312.1 p04003 q5vvq8 120830 samples serum plasma or cell culture supernatant assay type quantitative sandwich detection range 100 ng ml 1.56 sensitivity up to 0.5 intra precision component4 range100 to0.5
⇄⧉products_description => string (826) "Principle of the Assay: As mentioned above, this kit utilizes the Double Ant...
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Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Chicken C4 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
CD40/CD40L Signaling Pathway||138061!!Complement And Coagulation Cascades Pathway||83073!!Complement And Coagulation Cascades Pathway||484!!Complement Cascade Pathway||106405!!Immune System Pathway||106386!!Innate Immune System Pathway||106387!!Pertussis Pathway||218111!!Pertussis Pathway||218099!!Regulation Of Complement Cascade Pathway||576254
⇄sp_protein_name => string (31) "C4b-binding protein alpha chain"
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aaa12694 chicken no cross reaction with other factors typical testing data standard curve for reference only aaa12694_sc elisa kit complement component 4 c4 binding protein alpha c4bpa prp c4bp c4b chain proline rich 67,033 da c4bpa_human 18490166 aah22312.1 p04003 q5vvq8 120830 samples chichen serum plasma or cell culture supernatant assay type quantitative sandwich detection range 1000 ng ml 15.6 sensitivity up to 5 intra precision component4 to5
