Stk3 blocking peptide

Mouse Stk3 Antibody (C-term) Blocking Peptide

• Gene Names: Stk3; MST; Mst2; Mst3; Ste20; mess1; 0610042I06Rik
• Synonyms: Stk3; Mouse Stk3 Antibody (C-term) Blocking Peptide; Serine/threonine-protein kinase 3; Mammalian STE20-like protein kinase 2; MST-2; STE20-like kinase MST2; Serine/threonine-protein kinase 3 36kDa subunit; MST2/N; Serine/threonine-protein kinase 3 20kDa subunit; MST2/C; Mess1; Mst2; Stk3 blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 497

• Cellular Location: Cytoplasm. Nucleus. Note: The caspase-cleaved form cycles between nucleus and cytoplasm. Phosphorylation at Thr-117 leads to inhibition of nuclear translocation (By similarity)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Stk3 blocking peptide

Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1. Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosomes, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38 (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 46577548
• NCBI GeneID: 56274
• UniProt Accession #: Q9JI10; Q60877; Q80UG4; Q8CI58
• Molecular Weight: 49,067 Da
• NCBI Official Full Name: Serine/threonine-protein kinase 3
• NCBI Official Synonym Full Names: serine/threonine kinase 3
• NCBI Official Symbol: Stk3
• NCBI Official Synonym Symbols: MST; Mst2; Mst3; Ste20; mess1; 0610042I06Rik
• NCBI Protein Information: serine/threonine-protein kinase 3
• UniProt Protein Name: Serine/threonine-protein kinase 3
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: Stk3
• UniProt Synonym Gene Names: Mess1; Mst2; MST-2; MST2/N; MST2/C
• UniProt Entry Name: STK3_MOUSE

Uniprot Description

MST2: a protein kinase of the STE20 family. Activated by apoptotic signals as well as other stress conditions. Full activation requires both phosphorylation and caspase-mediated cleavage. Following cleavage by caspase-3, undergoes irreversible autophosphorylation. Full-length is mainly unphosphorylated in the rat thymus and cultured cells, whereas caspase-3-cleaved MST2 in apoptotic cells is highly phosphorylated.

Protein type: EC 2.7.11.1; Autophagy; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; STE group; STE20 family; MST subfamily

Cellular Component: cytoplasm; protein complex

Molecular Function: ATP binding; magnesium ion binding; protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: central nervous system development; negative regulation of cell proliferation; negative regulation of organ growth; neural tube formation; positive regulation of apoptosis; positive regulation of fat cell differentiation; positive regulation of JNK cascade; positive regulation of protein binding; positive regulation of protein kinase B signaling cascade; positive regulation of transcription factor activity; protein amino acid phosphorylation; protein stabilization