Prkcd blocking peptide

Mouse Prkcd Antibody (N-term) Blocking Peptide

• Gene Names: Prkcd; Pkcd; PKC[d]; AI385711; PKCdelta; D14Ertd420e
• Synonyms: Prkcd; Mouse Prkcd Antibody (N-term) Blocking Peptide; Protein kinase C delta type; Tyrosine-protein kinase PRKCD; nPKC-delta; Protein kinase C delta type regulatory subunit; Protein kinase C delta type catalytic subunit; Sphingosine-dependent protein kinase-1; SDK1; Pkcd; Prkcd blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 674

• Cellular Location: Cytoplasm. Cytoplasm, perinuclear region. Nucleus Endoplasmic reticulum. Mitochondrion Membrane; Peripheral membrane protein
• Tissue Location: Isoform 1 is highly expressed in developing pro- and pre-B-cells and moderately in mature T-cells. Isoform 2 is highly expressed in testis and ovary and at a lower level in thymocytes, brain and kidney
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Prkcd blocking peptide

Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays contrasting roles in cell death and cell survival by functioning as a pro-apoptotic protein during DNA damage-induced apoptosis, but acting as an anti-apoptotic protein during cytokine receptor- initiated cell death, is involved in tumor suppression, is required for oxygen radical production by NADPH oxidase and acts as positive or negative regulator in platelet functional responses. Negatively regulates B cell proliferation and also has an important function in self-antigen induced B cell tolerance induction. Upon DNA damage, activates the promoter of the death- promoting transcription factor BCLAF1/Btf to trigger BCLAF1- mediated p53/TP53 gene transcription and apoptosis. In response to oxidative stress, interact with and activate CHUK/IKKA in the nucleus, causing the phosphorylation of p53/TP53. In the case of ER stress or DNA damage-induced apoptosis, can form a complex with the tyrosine-protein kinase ABL1 which trigger apoptosis independently of p53/TP53. In cytosol can trigger apoptosis by activating MAPK11 or MAPK14, inhibiting AKT1 and decreasing the level of X-linked inhibitor of apoptosis protein (XIAP), whereas in nucleus induces apoptosis via the activation of MAPK8 or MAPK9. Upon ionizing radiation treatment, is required for the activation of the apoptosis regulators BAX and BAK, which trigger the mitochondrial cell death pathway. Can phosphorylate MCL1 and target it for degradation which is sufficient to trigger for BAX activation and apoptosis. Is required for the control of cell cycle progression both at G1/S and G2/M phases. Mediates phorbol 12-myristate 13-acetate (PMA)-induced inhibition of cell cycle progression at G1/S phase by up-regulating the CDK inhibitor CDKN1A/p21 and inhibiting the cyclin CCNA2 promoter activity. In response to UV irradiation can phosphorylate CDK1, which is important for the G2/M DNA damage checkpoint activation. Can protect glioma cells from the apoptosis induced by TNFSF10/TRAIL, probably by inducing increased phosphorylation and subsequent activation of AKT1. Can also act as tumor suppressor upon mitogenic stimulation with PMA or TPA. In N-formyl-methionyl- leucyl-phenylalanine (fMLP)-treated cells, is required for NCF1 (p47-phox) phosphorylation and activation of NADPH oxidase activity, and regulates TNF-elicited superoxide anion production in neutrophils, by direct phosphorylation and activation of NCF1 or indirectly through MAPK1/3 (ERK1/2) signaling pathways. May also play a role in the regulation of NADPH oxidase activity in eosinophil after stimulation with IL5, leukotriene B4 or PMA. In collagen-induced platelet aggregation, acts a negative regulator of filopodia formation and actin polymerization by interacting with and negatively regulating VASP phosphorylation. Downstream of PAR1, PAR4 and CD36/GP4 receptors, regulates differentially platelet dense granule secretion; acts as a positive regulator in PAR-mediated granule secretion, whereas it negatively regulates CD36/GP4-mediated granule release. Phosphorylates MUC1 in the C- terminal and regulates the interaction between MUC1 and beta- catenin. The catalytic subunit phosphorylates 14-3-3 proteins (YWHAB, YWHAZ and YWHAH) in a sphingosine-dependent fashion.

NCBI and Uniprot Product Information

• NCBI GI #: 20141533
• NCBI GeneID: 18753
• UniProt Accession #: P28867; Q91V85; Q9Z333
• Molecular Weight: 80,294 Da
• NCBI Official Full Name: Protein kinase C delta type
• NCBI Official Synonym Full Names: protein kinase C, delta
• NCBI Official Symbol: Prkcd
• NCBI Official Synonym Symbols: Pkcd; PKC[d]; AI385711; PKCdelta; D14Ertd420e
• NCBI Protein Information: protein kinase C delta type
• UniProt Protein Name: Protein kinase C delta type
• Protein Family: Protein kinase
• UniProt Gene Name: Prkcd
• UniProt Synonym Gene Names: Pkcd; SDK1
• UniProt Entry Name: KPCD_MOUSE

Uniprot Description

PKCD: an AGC kinase of the PKC family. A novel PKC: Ca2 independent but still regulated by PS, DAG and phorbol esters. Caspase-3 appears to liberate a sphingosine-activated catalytic fragment that has altered regulatory mechanisms and substrate specificities.

Protein type: EC 2.7.11.13; Protein kinase, Ser/Thr (non-receptor); Nuclear receptor co-regulator; Kinase, protein; Protein kinase, AGC; Tumor suppressor; AGC group; PKC family; Delta subfamily

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; intercellular junction; membrane; mitochondrion; nuclear matrix; nucleus; plasma membrane

Molecular Function: calcium-independent protein kinase C activity; enzyme activator activity; enzyme binding; insulin receptor substrate binding; kinase binding; protein binding; protein kinase activity; protein kinase binding; protein kinase C activity; protein serine/threonine kinase activity

Biological Process: activation of protein kinase activity; apoptosis; aspartate transport; B cell proliferation; collagen metabolic process; defense response to bacterium; histone phosphorylation; immunoglobulin mediated immune response; interleukin-10 production; interleukin-12 production; negative regulation of actin filament polymerization; negative regulation of filopodium formation; negative regulation of insulin receptor signaling pathway; negative regulation of MAP kinase activity; negative regulation of peptidyl-tyrosine phosphorylation; neutrophil activation; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of apoptosis; positive regulation of endodeoxyribonuclease activity; positive regulation of glucose import; positive regulation of MAP kinase activity; positive regulation of MAPKKK cascade; positive regulation of protein amino acid dephosphorylation; positive regulation of protein import into nucleus; positive regulation of superoxide release; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of actin cytoskeleton organization and biogenesis; regulation of phosphorylation; response to oxidative stress