GOLGA5 blocking peptide

GOLGA5 Antibody (Center) Blocking peptide

• Gene Names: GOLGA5; RFG5; GOLIM5; ret-II
• Synonyms: GOLGA5; GOLGA5 Antibody (Center) Blocking peptide; Golgin subfamily A member 5; Cell proliferation-inducing gene 31 protein; Golgin-84; Protein Ret-II; RET-fused gene 5 protein; RETII; RFG5; GOLGA5 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the Center region of GOLGA5. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 731

• Cellular Location: Golgi apparatus membrane; Single-pass type IV membrane protein. Note: Found throughout the Golgi, both on cisternae and, at higher abundance, on the tubulo- vesicular structures of the cis-Golgi network
• Tissue Location: Ubiquitous. Highly expressed in seminiferous tubules and Leydig cells in testis, and detected at much lower levels in the other tissues tested. Expression is very low or not detectable in spermatozoa.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for GOLGA5 blocking peptide

Involved in maintaining Golgi structure. Stimulates the formation of Golgi stacks and ribbons. Involved in intra-Golgi retrograde transport.

NCBI and Uniprot Product Information

• NCBI GI #: 296439337
• NCBI GeneID: 9950
• UniProt Accession #: Q8TBA6; O95287; Q03962; Q2TS49; Q9UQQ7; C9JRU1
• Molecular Weight: 78,108 Da
• NCBI Official Full Name: Golgin subfamily A member 5
• NCBI Official Synonym Full Names: golgin A5
• NCBI Official Symbol: GOLGA5
• NCBI Official Synonym Symbols: RFG5; GOLIM5; ret-II
• NCBI Protein Information: golgin subfamily A member 5
• UniProt Protein Name: Golgin subfamily A member 5
• Protein Family: Golgin subfamily
• UniProt Gene Name: GOLGA5
• UniProt Synonym Gene Names: RETII; RFG5
• UniProt Entry Name: GOGA5_HUMAN

NCBI Description

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues; the chimeric sequences have been designated RET-II and PTC5. A pseudogene of this gene is located on the short arm of chromosome 5. [provided by RefSeq, Jul 2013]

Uniprot Description

GOLGA5: a member of the golgin family of proteins, whose members localize to the Golgi. Involved in regulating the architecture of the Golgi apparatus. A coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues. Highly phosphorylated during mitosis. Phosphorylation is barely detectable during interphase. Two splice variant isoforms have been described.

Protein type: Cytoskeletal; Membrane protein, integral

Chromosomal Location of Human Ortholog: 14q32.12

Cellular Component: cis-Golgi network; Golgi apparatus; Golgi cisterna; Golgi membrane; integral to membrane; membrane; transport vesicle

Molecular Function: protein homodimerization activity; Rab GTPase binding

Biological Process: Golgi organization and biogenesis; Golgi vesicle transport; retrograde transport, vesicle recycling within Golgi

Disease: Thyroid Carcinoma, Papillary