SYT5 blocking peptide

SYT5 Antibody (Center) Blocking peptide

• Synonyms: SYT5; SYT5 Antibody (Center) Blocking peptide; Synaptotagmin-5; Synaptotagmin V; SytV; SYT5 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the Center region of SYT5. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 386

• Cellular Location: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass membrane protein. Recycling endosome membrane; Single-pass membrane protein. Note: In mast cells, localizes to the endocytic recycling compartment.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for SYT5 blocking peptide

May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Regulates the Ca(2+)- dependent secretion of norepinephrine in PC12 cells. Required for export from the endocytic recycling compartment to the cell surface (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 32700066
• NCBI GeneID: 6861
• UniProt Accession #: O00445; Q86X72; B3KWJ8; B7Z300
• Molecular Weight: 42,474 Da
• NCBI Official Full Name: Synaptotagmin-5
• NCBI Official Synonym Full Names: synaptotagmin 5
• NCBI Official Symbol: SYT5
• NCBI Protein Information: synaptotagmin-5
• UniProt Protein Name: Synaptotagmin-5
• Protein Family: Synaptotagmin
• UniProt Gene Name: SYT5
• UniProt Synonym Gene Names: SytV
• UniProt Entry Name: SYT5_HUMAN

NCBI Description

Synaptotagmins, such as SYT5, are a family of type III membrane proteins characterized by cytoplasmic repeats related to protein kinase C (see MIM 176960) regulatory (C2) domains, which are thought to bind calcium. Synaptotagmins may act both as negative regulators of vesicle fusion, allowing fusion in the presence of calcium, and as calcium receptors or sensor molecules (summary by Hudson and Birnbaum, 1995 [PubMed 7597049]).[supplied by OMIM, Feb 2011]

Uniprot Description

SYT5: May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Regulates the Ca(2+)- dependent secretion of norepinephrine in PC12 cells. Required for export from the endocytic recycling compartment to the cell surface. Belongs to the synaptotagmin family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.42|11p

Cellular Component: plasma membrane; synaptic vesicle membrane

Molecular Function: calcium ion binding; calcium-dependent phospholipid binding; clathrin binding; syntaxin binding

Biological Process: calcium ion-dependent exocytosis of neurotransmitter; regulation of calcium ion-dependent exocytosis; synaptic transmission; synaptic vesicle endocytosis; vesicle fusion