PDE11A blocking peptide

PDE11A Antibody (N-term) Blocking peptide

• Gene Names: PDE11A; PPNAD2
• Synonyms: PDE11A; PDE11A Antibody (N-term) Blocking peptide; Dual 3';5'-cyclic-AMP and -GMP phosphodiesterase 11A; cAMP and cGMP phosphodiesterase 11A; PDE11A blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of PDE11A. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 933

• Cellular Location: Cytoplasm, cytosol
• Tissue Location: Isoform 1 is present in prostate, pituitary, heart and liver. It is however not present in testis nor in penis, suggesting that weak inhibition by Tadalafil (Cialis) is not relevant (at protein level). Isoform 2 may be expressed in testis Isoform 4 is expressed in adrenal cortex
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for PDE11A blocking peptide

Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides cAMP and cGMP. Catalyzes the hydrolysis of both cAMP and cGMP to 5'-AMP and 5'- GMP, respectively.

NCBI and Uniprot Product Information

• NCBI GI #: 296439264
• NCBI GeneID: 50940
• UniProt Accession #: Q9HCR9; Q14CD1; Q53T16; Q96S76; Q9GZY7; Q9HB46; Q9NY45
• Molecular Weight: 55,700 Da
• NCBI Official Full Name: Dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11A
• NCBI Official Synonym Full Names: phosphodiesterase 11A
• NCBI Official Symbol: PDE11A
• NCBI Official Synonym Symbols: PPNAD2
• NCBI Protein Information: dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11A
• UniProt Protein Name: Dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11A
• Protein Family: Dual 3',5'-cyclic-AMP and -GMP phosphodiesterase
• UniProt Gene Name: PDE11A
• UniProt Entry Name: PDE11_HUMAN

NCBI Description

The 3',5'-cyclic nucleotides cAMP and cGMP function as second messengers in a wide variety of signal transduction pathways. 3',5'-cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of cAMP and cGMP to the corresponding 5'-monophosphates and provide a mechanism to downregulate cAMP and cGMP signaling. This gene encodes a member of the PDE protein superfamily. Mutations in this gene are a cause of Cushing disease and adrenocortical hyperplasia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PDE11A: Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides cAMP and cGMP. Catalyzes the hydrolysis of both cAMP and cGMP to 5'-AMP and 5'- GMP, respectively. Defects in PDE11A are the cause of primary pigmented nodular adrenocortical disease type 2 (PPNAD2). Primary pigmented nodular adrenocortical disease is a rare bilateral adrenal defect causing ACTH-independent Cushing syndrome. PPNAD2 is characterized by adrenal glands with overall normal size and weight, and multiple small yellow-to-dark brown nodules surrounded by a cortex with a uniform appearance. Microscopically, there are moderate diffuse cortical hyperplasia with mostly nonpigmented nodules, multiple capsular deficits and massive circumscribed and infiltrating extra-adrenal cortical excrescences with micronodules. PPNAD2 leads to Cushing syndrome. Belongs to the cyclic nucleotide phosphodiesterase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.4.17; Phosphodiesterase; Nucleotide Metabolism - purine; Mitochondrial; EC 3.1.4.35

Chromosomal Location of Human Ortholog: 2q31.2

Cellular Component: cytosol

Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; 3',5'-cyclic-GMP phosphodiesterase activity; 3',5'-cyclic-nucleotide phosphodiesterase activity; cGMP binding; cGMP-stimulated cyclic-nucleotide phosphodiesterase activity

Disease: Pigmented Nodular Adrenocortical Disease, Primary, 2