LAMB1 blocking peptide

LAMB1 Antibody (C-term) Blocking peptide

• Gene Names: LAMB1; CLM; LIS5
• Synonyms: LAMB1; LAMB1 Antibody (C-term) Blocking peptide; Laminin subunit beta-1; Laminin B1 chain; Laminin-1 subunit beta; Laminin-10 subunit beta; Laminin-12 subunit beta; Laminin-2 subunit beta; Laminin-6 subunit beta; Laminin-8 subunit beta; LAMB1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of LAMB1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 1786

• Cellular Location: Secreted, extracellular space, extracellular matrix, basement membrane. Note: Major component
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for LAMB1 blocking peptide

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Involved in the organization of the laminar architecture of cerebral cortex. It is probably required for the integrity of the basement membrane/glia limitans that serves as an anchor point for the endfeet of radial glial cells and as a physical barrier to migrating neurons. Radial glial cells play a central role in cerebral cortical development, where they act both as the proliferative unit of the cerebral cortex and a scaffold for neurons migrating toward the pial surface.

NCBI and Uniprot Product Information

• NCBI GI #: 317373377
• NCBI GeneID: 3912
• UniProt Accession #: P07942; Q14D91
• Molecular Weight: 198,038 Da
• NCBI Official Full Name: Laminin subunit beta-1
• NCBI Official Synonym Full Names: laminin subunit beta 1
• NCBI Official Symbol: LAMB1
• NCBI Official Synonym Symbols: CLM; LIS5
• NCBI Protein Information: laminin subunit beta-1
• UniProt Protein Name: Laminin subunit beta-1
• Protein Family: Maltoporin
• UniProt Gene Name: LAMB1
• UniProt Entry Name: LAMB1_HUMAN

NCBI Description

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin, beta 1. The beta 1 chain has 7 structurally distinct domains which it shares with other beta chain isomers. The C-terminal helical region containing domains I and II are separated by domain alpha, domains III and V contain several EGF-like repeats, and domains IV and VI have a globular conformation. Laminin, beta 1 is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. A sequence in the beta 1 chain that is involved in cell attachment, chemotaxis, and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis. [provided by RefSeq, Aug 2011]

Uniprot Description

LAMB1: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: basement membrane; extracellular matrix; extracellular region; extracellular space; laminin-1 complex; laminin-10 complex; laminin-2 complex; laminin-8 complex; perinuclear region of cytoplasm

Molecular Function: extracellular matrix structural constituent

Biological Process: cell adhesion; extracellular matrix organization and biogenesis; neurite development; neuronal-glial interaction involved in cerebral cortex radial glia guided migration; odontogenesis; positive regulation of cell migration; positive regulation of epithelial cell proliferation

Disease: Lissencephaly 5