NLRP10 blocking peptide

NLRP10 Antibody (C-term) Blocking peptide

• Gene Names: NLRP10; NOD8; PAN5; PYNOD; NALP10; CLR11.1
• Synonyms: NLRP10; NLRP10 Antibody (C-term) Blocking peptide; NACHT; LRR and PYD domains-containing protein 10; Nucleotide-binding oligomerization domain protein 8; NALP10; NOD8; PYNOD; NLRP10 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of NLRP10. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 655

• Cellular Location: Cytoplasm. Cell membrane. Note: Cytoplasmic protein which is recruited to the cell membrane by NOD1 following invasive bacterial infection
• Tissue Location: Highly expressed in basal and suprabasal epidermal cell layers with lower levels in dermal fibroblast cells (at protein level). Widely expressed with highest levels in heart, brain and skeletal muscle. Also expressed in liver, colon, dermis and epidermis. Little expression detected in myeloid cells or peripheral blood mononuclear cells.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for NLRP10 blocking peptide

Inhibits autoprocessing of CASP1, CASP1-dependent IL1B secretion, PYCARD aggregation and PYCARD-mediated apoptosis but not apoptosis induced by FAS or BID. Displays anti-inflammatory activity. Plays a role in adaptive immunity through control of dendritic cell-mediated transport of antigen to the lymph nodes from peripheral sites. Required for immunity against C.albicans infection. Involved in the innate immune response by contributing to proinflammatory cytokine release in response to invasive bacterial infection.

NCBI and Uniprot Product Information

• NCBI GI #: 46396380
• NCBI GeneID: 338322
• UniProt Accession #: Q86W26; Q2M3C4; Q6JGT0
• Molecular Weight: 75,032 Da
• NCBI Official Full Name: NACHT, LRR and PYD domains-containing protein 10
• NCBI Official Synonym Full Names: NLR family pyrin domain containing 10
• NCBI Official Symbol: NLRP10
• NCBI Official Synonym Symbols: NOD8; PAN5; PYNOD; NALP10; CLR11.1
• NCBI Protein Information: NACHT, LRR and PYD domains-containing protein 10
• UniProt Protein Name: NACHT, LRR and PYD domains-containing protein 10
• Protein Family: NACHT, LRR and PYD domains-containing protein
• UniProt Gene Name: NLRP10
• UniProt Synonym Gene Names: NALP10; NOD8; PYNOD
• UniProt Entry Name: NAL10_HUMAN

NCBI Description

Members of the NALP protein family typically contain a NACHT domain, a NACHT-associated domain (NAD), a C-terminal leucine-rich repeat (LRR) region, and an N-terminal pyrin domain (PYD). The protein encoded by this gene belongs to the NALP protein family despite lacking the LRR region. This protein likely plays a regulatory role in the innate immune system. The protein belongs to the signal-induced multiprotein complex, the inflammasome, that activates the pro-inflammatory caspases, caspase-1 and caspase-5. Other experiments indicate that this gene acts as a multifunctional negative regulator of inflammation and apoptosis. [provided by RefSeq, Jul 2008]

Uniprot Description

NALP10: Inhibits CASP1/caspase-1-dependent IL1B secretion and PYCARD-mediated apoptosis, but not that induced by FAS or BID. Belongs to the NLRP family.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: cytoplasm; extrinsic to plasma membrane

Biological Process: defense response to fungus; defense response to Gram-negative bacterium; positive regulation of T-helper 1 type immune response