PLD6 blocking peptide

PLD6 Antibody (Center) Blocking peptide

• Gene Names: PLD6; ZUC
• Synonyms: PLD6; PLD6 Antibody (Center) Blocking peptide; Mitochondrial cardiolipin hydrolase; 31--; Choline phosphatase 6; Mitochondrial phospholipase; MitoPLD; Phosphatidylcholine-hydrolyzing phospholipase D6; Phospholipase D6; PLD 6; Protein zucchini homolog; PLD6 blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 252

• Cellular Location: Mitochondrion outer membrane; Single-pass membrane protein
• Tissue Location: Widely expressed. Expression is not limited to testis and ovary.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for PLD6 blocking peptide

Regulates mitochondrial shape through facilitating mitochondrial fusion. During spermatogenesis, plays a critical role in PIWI-interacting RNA (piRNA) biogenesis (By similarity). piRNAs provide essential protection against the activity of mobile genetic elements. piRNA-mediated transposon silencing is thus critical for maintaining genome stability, in particular in germline cells when transposons are mobilized as a consequence of wide-spread genomic demethylation. Has been shown to be a backbone-non-specific, single strand-specific nuclease, cleaving either RNA or DNA substrates with similar affinity (By similarity). Produces 5' phosphate and 3' hydroxyl termini, suggesting it could directly participate in the processing of primary piRNA transcripts (By similarity). Has been proposed to act as a cardiolipin hydrolase to generate phosphatidic acid at mitochondrial surface. Although it cannot be excluded that it can act as a phospholipase in some circumstances, it should be noted that cardiolipin hydrolase activity is either undetectable in vitro, or very low (PubMed:21397848). In addition, cardiolipin is almost exclusively found on the inner mitochondrial membrane, while PLD6 localizes to the outer mitochondrial membrane, facing the cytosol.

NCBI and Uniprot Product Information

• NCBI GI #: 30520348
• NCBI GeneID: 201164
• NCBI Accession #: NP_849158.1
• NCBI GenBank Nucleotide #: NM_178836.2
• UniProt Accession #: Q8N2A8; Q8N5Y1
• Molecular Weight: 28,273 Da
• NCBI Official Full Name: phospholipase D6
• NCBI Official Synonym Full Names: phospholipase D family member 6
• NCBI Official Symbol: PLD6
• NCBI Official Synonym Symbols: ZUC
• NCBI Protein Information: mitochondrial cardiolipin hydrolase
• UniProt Protein Name: Mitochondrial cardiolipin hydrolase
• Protein Family: Mitochondrial cardiolipin hydrolase
• UniProt Gene Name: PLD6
• UniProt Synonym Gene Names: PLD 6
• UniProt Entry Name: PLD6_HUMAN

Uniprot Description

PLD6: Cardiolipin hydrolase present at the mitochondrial outer membrane required both for mitochondrial fusion and piRNA metabolic process. Acts by catalyzing the hydrolysis of cardiolipin (diphosphatidylglycerol) to form phosphatidate (phosphatidic acid or PA) at the mitochondrial outer membrane surface, promoting mitochondrial fusion. The production of phosphatidate also regulates the piRNA metabolic process by promoting recruitment and/or activation of components of the meiotic nuage, also named P granule, a critical step for primary biogenesis of piRNAs. Required during spermatogenesis to repress transposable elements and prevent their mobilization via its role in the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Belongs to the phospholipase D family. MitoPLD/Zucchini subfamily.

Protein type: EC 3.1.4.-; EC 3.1.4.4; Membrane protein, integral; Phospholipase

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: mitochondrial outer membrane

Molecular Function: cardiolipin synthase activity; protein binding; protein homodimerization activity

Biological Process: cardiolipin biosynthetic process; DNA methylation during gametogenesis; meiosis; mitochondrial fusion; polar granule organization and biogenesis; spermatid development