Rabbit CASK Polyclonal Antibody | anti-CASK antibody

CASK Antibody (Center K227)

Cat. Num. AAA9217136

• Gene Names: CASK; CMG; FGS4; LIN2; TNRC8; CAGH39; CAMGUK; MICPCH; MRXSNA
• Reactivity: Human; Predicted Reactivity: Mouse, Rat
• Applications: Western Blot, ELISA
• Synonyms: CASK; Polyclonal Antibody; CASK Antibody (Center K227); Peripheral plasma membrane protein CASK; hCASK; Calcium/calmodulin-dependent serine protein kinase; Protein lin-2 homolog; LIN2; anti-CASK antibody

• Host: Rabbit
• Reactivity: Human; Predicted Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This CASK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 262-291 amino acids from the Central region of human CASK.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Length: 898

• Applicable Applications for anti-CASK antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB:~~1:1000
• Subtitle: Purified Rabbit Polyclonal Antibody (Pab)
• Cellular Location: Nucleus. Cytoplasm. Cell membrane; Peripheral membrane protein.
• Tissue Location: Ubiquitous. Expression is significantly greater in brain relative to kidney, lung, and liver and in fetal brain and kidney relative to lung and liver.
• Antigen Region: 262-291 aa
• Antigen Type: Synthetic Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-CASK (Center K227) Antibody at 1:1000 dilution + SH-SY5Y whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 105 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Western blot analysis of CASK (Center K227) in Hela cell line lysates (35ug/lane).CASK (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of CASK (Center K227) in HL-60 cell line lysates (35ug/lane).CASK (arrow) was detected using the purified Pab.)

Related Product Information for anti-CASK antibody

Description: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The calcium/calmodulin-dependent kinase (CAMK) group consists of 75 kinases regulated by Ca2+/CaM and close relative family (CAMK, CAMKL, DAPK, MAPKAPK).

Background: Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TBR1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4. 1.

Product Categories/Family for anti-CASK antibody

Primary Antibodies; Neuroscience; Signal Transduction

References

Stevenson, D., et al., Mamm. Genome 11 (10):934-937 (2000). Cohen, A.R., et al., J. Cell Biol. 142 (1):129-138 (1998). Daniels, D.L., et al., Nat. Struct. Biol. 5 (4):317-325 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 186700627
• NCBI GeneID: 8573
• NCBI Accession #: NP_001119526.1
• NCBI GenBank Nucleotide #: NM_001126054.2
• UniProt Accession #: O14936; O43215; Q17RI4; Q59HA0; Q5VT16; Q5VT17; Q5VT18; Q5VT19; Q66T42; Q9BYH6; A6NES1; B7ZKY0
• Molecular Weight: 105123 Da
• NCBI Official Full Name: peripheral plasma membrane protein CASK isoform 2
• NCBI Official Synonym Full Names: calcium/calmodulin dependent serine protein kinase
• NCBI Official Symbol: CASK
• NCBI Official Synonym Symbols: CMG; FGS4; LIN2; TNRC8; CAGH39; CAMGUK; MICPCH; MRXSNA
• NCBI Protein Information: peripheral plasma membrane protein CASK
• UniProt Protein Name: Peripheral plasma membrane protein CASK
• Protein Family: Caskin
• UniProt Gene Name: CASK
• UniProt Synonym Gene Names: LIN2; hCASK
• UniProt Entry Name: CSKP_HUMAN

NCBI Description

This gene encodes a calcium/calmodulin-dependent serine protein kinase. The encoded protein is a MAGUK (membrane-associated guanylate kinase) protein family member. These proteins are scaffold proteins and the encoded protein is located at synapses in the brain. Mutations in this gene are associated with FG syndrome 4, mental retardation and microcephaly with pontine and cerebellar hypoplasia, and a form of X-linked mental retardation. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]

Uniprot Description

CASK: a MAGUK family protein and a protein kinase of the CAMK group. Contains one protein kinase domain, one guanylate kinase domain, two L27 domains, one PDZ domain and one SH3 domain. Plays a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1. Lacks the canonical DFG motif required for Mg++ binding, but retains protein kinase activity even without binding Mg++. Six alternatively spliced human isoforms have been described.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Adaptor/scaffold; Protein kinase, CAMK; EC 2.7.11.1; CAMK group; CASK family

Chromosomal Location of Human Ortholog: Xp11.4

Cellular Component: actin cytoskeleton; basement membrane; basolateral plasma membrane; cytoplasm; cytosol; focal adhesion; intercellular junction; nuclear lamina; nuclear matrix; nucleolus; plasma membrane; presynaptic membrane; vesicle

Molecular Function: ATP binding; calmodulin binding; guanylate kinase activity; neurexin binding; protein binding; protein serine/threonine kinase activity

Biological Process: cell adhesion; GDP metabolic process; GMP metabolic process; negative regulation of cell-matrix adhesion; neurotransmitter secretion; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation

Disease: Fg Syndrome 4; Mental Retardation And Microcephaly With Pontine And Cerebellar Hypoplasia