CASK Kinase Recombinant Protein | CASK recombinant protein

Recombinant Human CASK Kinase Protein

Cat. Num. AAA2545638

• Gene Names: CASK; CMG; FGS4; LIN2; TNRC8; CAGH39; CAMGUK; MICPCH; MRXSNA
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: CASK Kinase; Recombinant Human CASK Kinase Protein; CASK; CASK recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4
• Sequence Length: 898

• Application Notes: The secreted recombinant human CASK consists of 899 amino acids and predicts a molecular mass of 102.1 KDa. The apparent molecular mass of the protein is approximately 102 KDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Gly
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CASK recombinant protein

Background: Peripheral plasma membrane protein CASK, also known as calcium/calmodulin-dependent serine protein kinase, CASK and LIN2, is a nucleus, cytoplasm and cell membrane protein which belongs to the MAGUK family. CASK / LIN2 contains one guanylate kinase-like domain, two L27 domains, one PDZ (DHR) domain, one protein kinase domain and one SH3 domain. CASK / LIN2 is ubiquitously expressed. Expression of CASK / LIN2 is significantly greater in brain relative to kidney, lung, and liver and in fetal brain and kidney relative to lung and liver. CASK / LIN2 is a multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. CASK / LIN2 contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. It binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. CASK / LIN2 may mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1. Defects in CASK are the cause of mental retardation X-linked CASK-related (MRXCASK). Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Defects in CASK are also the cause of FG syndrome type 4 which is an X-linked disorder characterized by mental retardation, relative macrocephaly, hypotonia and constipation.

Description: A DNA sequence encoding the human CASK (O14936-4) (Ala 2-Tyr 898) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 186700627
• NCBI GeneID: 8573
• NCBI Accession #: NP_001119526.1
• NCBI GenBank Nucleotide #: NM_001126054.2
• UniProt Accession #: O14936; O43215; Q17RI4; Q59HA0; Q5VT16; Q5VT17; Q5VT18; Q5VT19; Q66T42; Q9BYH6; A6NES1; B7ZKY0
• Molecular Weight: 103,211 Da
• NCBI Official Full Name: peripheral plasma membrane protein CASK isoform 2
• NCBI Official Synonym Full Names: calcium/calmodulin dependent serine protein kinase
• NCBI Official Symbol: CASK
• NCBI Official Synonym Symbols: CMG; FGS4; LIN2; TNRC8; CAGH39; CAMGUK; MICPCH; MRXSNA
• NCBI Protein Information: peripheral plasma membrane protein CASK
• UniProt Protein Name: Peripheral plasma membrane protein CASK
• Protein Family: Peripheral plasma membrane protein
• UniProt Gene Name: CASK
• UniProt Synonym Gene Names: LIN2; hCASK

NCBI Description

This gene encodes a calcium/calmodulin-dependent serine protein kinase. The encoded protein is a MAGUK (membrane-associated guanylate kinase) protein family member. These proteins are scaffold proteins and the encoded protein is located at synapses in the brain. Mutations in this gene are associated with FG syndrome 4, mental retardation and microcephaly with pontine and cerebellar hypoplasia, and a form of X-linked mental retardation. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]

Uniprot Description

CASK: a MAGUK family protein and a protein kinase of the CAMK group. Contains one protein kinase domain, one guanylate kinase domain, two L27 domains, one PDZ domain and one SH3 domain. Plays a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1. Lacks the canonical DFG motif required for Mg++ binding, but retains protein kinase activity even without binding Mg++. Six alternatively spliced human isoforms have been described.

Protein type: Adaptor/scaffold; CAMK group; CASK family; EC 2.7.11.1; Kinase, protein; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: Xp11.4

Cellular Component: actin cytoskeleton; basement membrane; cytoplasm; cytosol; focal adhesion; intercellular junction; nuclear lamina; nuclear matrix; nucleolus; plasma membrane; presynaptic membrane

Molecular Function: guanylate kinase activity; protein binding

Biological Process: cell adhesion; negative regulation of cell-matrix adhesion; neurotransmitter secretion

Disease: Fg Syndrome 4; Mental Retardation And Microcephaly With Pontine And Cerebellar Hypoplasia