Western Blot (WB)
(All lanes : Anti-Ascl1 (Human C-term) at 1:1000 dilutionLane 1: human brain lysateLane 2: A549 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit ASCL1 Polyclonal Antibody | anti-ASCL1 antibody
ASCL1 (Achaete-scute homolog 1) Antibody (C-term D220)
Western Blot (WB)
(All lanes : Anti-Ascl1 (Human C-term) at 1:1000 dilutionLane 1: human brain lysateLane 2: A549 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-Ascl1 (Human C-term) at 1:1000 dilutionLane 1: human brain lysateLane 2: A549 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western blot analysis of anti-Ascl1 Antibody (C-term D220) in Hela cell line lysates (35ug/lane). Ascl1(arrow) was detected using the purified Pab (1:160 dilution).)
Western Blot (WB)
(Western blot analysis of anti-Ascl1 Antibody (C-term D220) in Hela cell line lysates (35ug/lane). Ascl1(arrow) was detected using the purified Pab (1:160 dilution).)
Western Blot (WB)
(Western blot analysis of Ascl1 (arrow) using rabbit polyclonal Ascl1 Antibody (Human C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Ascl1 gene (Lane 2) (Origene Technologies).)
Western Blot (WB)
(Western blot analysis of Ascl1 (arrow) using rabbit polyclonal Ascl1 Antibody (Human C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Ascl1 gene (Lane 2) (Origene Technologies).)
Background: Transcription factor that controls transcriptional expression of its target genes by binding to the E box (5'-CANNTG- 3'). Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Essential for the generation of olfactory and autonomic neurons (By similarity).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases. [provided by RefSeq, Jul 2008]
Uniprot Description
ASCL1: Transcriptional regulator. May play a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Involved in the initiation of neuronal differentiation. Mediates transcription activation by binding to the E box (5'-CANNTG-3').
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 12q23.2
Cellular Component: cell soma; nucleus
Molecular Function: bHLH transcription factor binding; chromatin binding; DNA binding; protein binding; protein homodimerization activity; transcription factor activity
Biological Process: cell maturation; central nervous system neuron development; cerebral cortex development; cerebral cortex GABAergic interneuron differentiation; commitment of a neuronal cell to a specific type of neuron in the forebrain; musculoskeletal movement, spinal reflex action; negative regulation of apoptosis; negative regulation of neuron differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; neuroblast fate determination; neuroblast proliferation; neurogenesis; neuron fate specification; neuron migration; Notch signaling pathway; olfactory pit development; oligodendrocyte development; positive regulation of cell cycle; positive regulation of neuron apoptosis; positive regulation of neuron differentiation; positive regulation of Notch signaling pathway; positive regulation of transcription from RNA polymerase II promoter; regulation of epithelial cell differentiation; regulation of gene expression; regulation of mitotic cell cycle; regulation of timing of subpallium neuron differentiation; response to folic acid; response to lithium ion; response to retinoic acid; spinal cord association neuron differentiation; spinal cord oligodendrocyte cell fate specification; subpallium neuron fate commitment; sympathetic nervous system development; transcription, DNA-dependent; vestibular nucleus development
Disease: Central Hypoventilation Syndrome, Congenital
Research Articles on ASCL1
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Product Notes
The ASCL1 ascl1 (Catalog #AAA9217077) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ASCL1 (Achaete-scute homolog 1) Antibody (C-term D220) reacts with Human; Predicted Reactivity: Mouse, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's ASCL1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB:~~1:1000. Researchers should empirically determine the suitability of the ASCL1 ascl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ASCL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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