Western Blot (WB)
(Anti-HRH4 Antibody (C-term) at 1:2000 dilution + human heart lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human HRH4 Polyclonal Antibody | anti-H4 antibody
HRH4 Antibody (C-term)
Reactivity
Human
Applications
Western Blot
Synonyms
HRH4; Polyclonal Antibody; HRH4 Antibody (C-term); Purified Rabbit Polyclonal Antibody (Pab); Histamine receptor H4;; anti-H4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Sequence Length
390
Applicable Applications for anti-H4 antibody
Western Blot (WB)
Application Notes
WB~~1:2000
Antigen Type
Synthetic Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Anti-HRH4 Antibody (C-term) at 1:2000 dilution + human heart lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-HRH4 Antibody (C-term) at 1:2000 dilution + human heart lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western blot analysis of HRH4 Antibody (C-term) in 293 cell line lysates (35ug/lane). HRH4 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of HRH4 Antibody (C-term) in 293 cell line lysates (35ug/lane). HRH4 (arrow) was detected using the purified Pab.)
Related Product Information for anti-H4 antibody
Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H4 belongs to the family 1 of G protein-coupled receptors and has the highest homology to histamine receptor H3 among known G protein-coupled receptors.
References
Lippert, U., et al., J. Invest. Dermatol. 123(1):116-123 (2004). Coge, F., et al., Biochem. Biophys. Res. Commun. 284(2):301-309 (2001). Morse, K.L., et al., J. Pharmacol. Exp. Ther. 296(3):1058-1066 (2001). Zhu, Y., et al., Mol. Pharmacol. 59(3):434-441 (2001). Liu, C., et al., Mol. Pharmacol. 59(3):420-426 (2001).
NCBI and Uniprot Product Information
Similar Products
Product Notes
The H4 h4 (Catalog #AAA9216924) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HRH4 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HRH4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB~~1:2000. Researchers should empirically determine the suitability of the H4 h4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HRH4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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