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Western Blot (WB) (All lanes : Anti-PRSS21 Antibody (N-Term) at 1:1000-1:2000 dilutionLane 1: K562 whole cell lysatesLane 2: Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 35 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Human PRSS21 Polyclonal Antibody | anti-PRSS21 antibody

PRSS21 Antibody (N-Term)

Gene Names
PRSS21; ESP1; ESP-1; TEST1; TESTISIN
Reactivity
Human
Applications
Western Blot
Synonyms
PRSS21; Polyclonal Antibody; PRSS21 Antibody (N-Term); Purified Rabbit Polyclonal Antibody (Pab); Testisin; 3421-; Eosinophil serine protease 1; ESP-1; Serine protease 21; ESP1; TEST1; anti-PRSS21 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Ig
Specificity
This PRSS21 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 14-26 amino acids from the N-Term region of human PRSS21.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
225
Applicable Applications for anti-PRSS21 antibody
Western Blot (WB)
Application Notes
WB ~~ 1:1000-1:2000
Antigen Source
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-PRSS21 Antibody (N-Term) at 1:1000-1:2000 dilutionLane 1: K562 whole cell lysatesLane 2: Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 35 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-PRSS21 Antibody (N-Term) at 1:1000-1:2000 dilutionLane 1: K562 whole cell lysatesLane 2: Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 35 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-PRSS21 antibody
Could regulate proteolytic events associated with testicular germ cell maturation.
Product Categories/Family for anti-PRSS21 antibody
References
Inoue M.,et al.Biochem. Biophys. Res. Commun. 252:307-312(1998). Inoue M.,et al.Biochem. Biophys. Res. Commun. 266:564-568(1999). Hooper J.D.,et al.Cancer Res. 59:3199-3205(1999). Hooper J.D.,et al.Biochim. Biophys. Acta 1492:63-71(2000). Clark H.F.,et al.Genome Res. 13:2265-2270(2003).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34884
NCBI Official Full Name
testisin isoform 4 preproprotein
NCBI Official Synonym Full Names
protease, serine 21
NCBI Official Symbol
PRSS21
NCBI Official Synonym Symbols
ESP1; ESP-1; TEST1; TESTISIN
NCBI Protein Information
testisin
UniProt Protein Name
Testisin
Protein Family
UniProt Gene Name
PRSS21
UniProt Synonym Gene Names
ESP1; TEST1; ESP-1
UniProt Entry Name
TEST_HUMAN

NCBI Description

This gene encodes a cell-surface anchored serine protease, which is a member of the trypsin family of serine proteases. The encoded protein is predicted to be active on peptide linkages involving the carboxyl group of lysine or arginine. The encoded protein localizes to the cytoplasm and the plasma membrane of premeiotic testicular germ cells and may be involved in progression of testicular tumors of germ cell origin. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

PRSS21: Could regulate proteolytic events associated with testicular germ cell maturation. Belongs to the peptidase S1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.-; Membrane protein, GPI anchor; Protease

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: membrane; cytoplasm; plasma membrane

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: spermatogenesis; proteolysis

Research Articles on PRSS21

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Product Notes

The PRSS21 prss21 (Catalog #AAA9216694) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRSS21 Antibody (N-Term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRSS21 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB ~~ 1:1000-1:2000. Researchers should empirically determine the suitability of the PRSS21 prss21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRSS21, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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