Western Blot (WB)
(All lanes : Anti-Dnmt3b Antibody (Center) at 1:2000 dilutionLane 1: F9 whole cell lysatesLane 2: NIH/3T3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 97 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Mouse Dnmt3b Polyclonal Antibody | anti-Dnmt3b antibody
Mouse Dnmt3b Antibody (Center)
Western Blot (WB)
(All lanes : Anti-Dnmt3b Antibody (Center) at 1:2000 dilutionLane 1: F9 whole cell lysatesLane 2: NIH/3T3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 97 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-Dnmt3b Antibody (Center) at 1:2000 dilutionLane 1: F9 whole cell lysatesLane 2: NIH/3T3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 97 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
NCBI and Uniprot Product Information
NCBI Description
This is one of two related genes encoding de novo DNA methyltransferases, which are responsible for the establishment of DNA methylation patterns in embryos. Loss of function of this gene results in severe developmental defects and loss of viability. Mutation of the related gene in humans causes immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. There is a pseudogene for this gene located adjacent to this gene in the same region of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Nov 2012]
Uniprot Description
DNMT3B: a ubiquitous DNA methyltransferase required for genome wide de novo methylation and essential for development. DNA methylation is coordinated with methylation of histones. Numerous cancer cell lines and primary acute leukemias express aberrant DNMT3B transcripts, especially DNMT3B7. Transfection of DNMT3B7 into 293 cells alters the pattern of genes expressed in the transfected cells. Can interact with DNMT1, which might be a co-operative event during DNA methylation. Methylates CpG sites at a rate slower than DNMT3A and much slower than DNMT1. Interacts with SUV39H1, SETDB1, SUMO1 and UBE2I9. Interacts with DNMT1 and DNMT3A. Mutations in DNMT3B have been shown to cause immunodeficiency-centromeric instability-facial anomalies syndrome (ICF). Six alternatively spliced isoforms of the human protein have been reported. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate. Isoforms 4 and 5 are probably not enzymatically active due to the deletion of two conserved methyltransferase motifs.
Protein type: Methyltransferase, DNA; Cell development/differentiation; Amino Acid Metabolism - cysteine and methionine; EC 2.1.1.37; Methyltransferase
Cellular Component: nucleoplasm; heterochromatin; intracellular membrane-bound organelle; cytoplasm; nuclear heterochromatin; nucleus; chromosome, pericentric region
Molecular Function: transferase activity; DNA (cytosine-5-)-methyltransferase activity; methyltransferase activity; protein binding; DNA binding; unmethylated CpG binding; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; metal ion binding; histone deacetylase binding; chromatin binding; transcription corepressor activity; DNA-methyltransferase activity
Biological Process: methylation; negative regulation of histone H3-K9 methylation; genetic imprinting; S-adenosylhomocysteine metabolic process; negative regulation of transcription from RNA polymerase II promoter; positive regulation of histone H3-K4 methylation; cytosine methylation within a CG sequence; protein complex localization; methylation-dependent chromatin silencing; negative regulation of gene expression, epigenetic; regulation of gene expression; DNA methylation; S-adenosylmethioninamine metabolic process; positive regulation of neuron differentiation; negative regulation of transcription, DNA-dependent
Research Articles on Dnmt3b
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Product Notes
The Dnmt3b dnmt3b (Catalog #AAA9216213) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Mouse Dnmt3b Antibody (Center) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Dnmt3b can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB ~~ 1:2000. Researchers should empirically determine the suitability of the Dnmt3b dnmt3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dnmt3b, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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