Rabbit anti-Human HOOK2 Polyclonal Antibody | anti-HOOK2 antibody

HOOK2 Antibody (N-term)

Cat. Num. AAA9216082

• Gene Names: HOOK2; HK2
• Reactivity: Human
• Applications: Western Blot
• Synonyms: HOOK2; Polyclonal Antibody; HOOK2 Antibody (N-term); Purified Rabbit Polyclonal Antibody (Pab); Protein Hook homolog 2; h-hook2; hHK2; anti-HOOK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This HOOK2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 162-197 amino acids from the N-terminal region of human HOOK2.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 717

• Applicable Applications for anti-HOOK2 antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:2000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-HOOK2 Antibody (N-term)at 1:2000 dilution + MCF-7 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 83 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-HOOK2 antibody

Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). Contributes to the establishment and maintenance of centrosome function. May function in the positioning or formation of aggresomes, which are pericentriolar accumulations of misfolded proteins, proteasomes and chaperones.

Product Categories/Family for anti-HOOK2 antibody

Crown Antibodies

References

Kraemer H.,et al.Genetics 151:675-684(1999). Grimwood J.,et al.Nature 428:529-535(2004). Walenta J.H.,et al.J. Cell Biol. 152:923-934(2001). Szebenyi G.,et al.BMC Cell Biol. 8:19-19(2007). Szebenyi G.,et al.Traffic 8:32-46(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 154354987
• NCBI GeneID: 29911
• NCBI Accession #: NP_001093646.1
• NCBI GenBank Nucleotide #: NM_001100176.1
• UniProt Accession #: Q96ED9; O60562
• Molecular Weight: 83207
• NCBI Official Full Name: protein Hook homolog 2 isoform 2
• NCBI Official Synonym Full Names: hook microtubule-tethering protein 2
• NCBI Official Symbol: HOOK2
• NCBI Official Synonym Symbols: HK2
• NCBI Protein Information: protein Hook homolog 2
• UniProt Protein Name: Protein Hook homolog 2
• Protein Family: Protein Hook
• UniProt Gene Name: HOOK2
• UniProt Synonym Gene Names: h-hook2; hHK2
• UniProt Entry Name: HOOK2_HUMAN

NCBI Description

Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. The Drosophila Hook protein is a component of the endocytic compartment.[supplied by OMIM, Apr 2004]

Uniprot Description

HOOK2: Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). Contributes to the establishment and maintenance of centrosome function. May function in the positioning or formation of aggresomes, which are pericentriolar accumulations of misfolded proteins, proteasomes and chaperones. Belongs to the hook family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: microtubule; centrosome

Molecular Function: identical protein binding; protein binding

Biological Process: protein transport; endosome organization and biogenesis; lysosome organization and biogenesis; early endosome to late endosome transport; endosome to lysosome transport; endocytosis