Rabbit anti-Human ECI1 Polyclonal Antibody | anti-ECI1 antibody

ECI1 Antibody

Cat. Num. AAA9215647

• Gene Names: ECI1; DCI
• Reactivity: Human
• Applications: Western Blot
• Synonyms: ECI1; Polyclonal Antibody; ECI1 Antibody; Purified Rabbit Polyclonal Antibody (Pab); Enoyl-CoA delta isomerase 1; mitochondrial; 5.3.3.8; 3; 2-trans-enoyl-CoA isomerase; Delta(3); Delta(2)-enoyl-CoA isomerase; D3; D2-enoyl-CoA isomerase; Dodecenoyl-CoA isomerase; DCI; anti-ECI1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Form/Format: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Sequence Length: 285

• Applicable Applications for anti-ECI1 antibody: Western Blot (WB)
• Application Notes: WB ~~1:1000
• Antigen Source: Human
• Antigen Type: A Synthesized Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-ECI1 Antibody at 1:1000 dilutionLane 1: 293T/17 whole cell lysateLane 2: NCI-H1299 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 33 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-ECI1 antibody

Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species.

Product Categories/Family for anti-ECI1 antibody

Cancer; Cardiovascular; Metabolism; Signal transduction

References

Janssen U.,et al.Genomics 23:223-228(1994). Ota T.,et al.Nat. Genet. 36:40-45(2004). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. Kilponen J.M.,et al.Biochem. J. 300:1-5(1994). Takahashi Y.,et al.Biochem. J. 304:849-852(1994).

NCBI and Uniprot Product Information

• NCBI GI #: 295842266
• NCBI GeneID: 1632
• NCBI Accession #: NP_001171500.1
• NCBI GenBank Nucleotide #: NM_001178029.1
• UniProt Accession #: P42126; Q13290; Q7Z2L6; Q7Z2L7; Q9BUB8; Q9BW05; Q9UDG6; A8K512
• Molecular Weight: 32816
• NCBI Official Full Name: enoyl-CoA delta isomerase 1, mitochondrial isoform 2
• NCBI Official Synonym Full Names: enoyl-CoA delta isomerase 1
• NCBI Official Symbol: ECI1
• NCBI Official Synonym Symbols: DCI
• NCBI Protein Information: enoyl-CoA delta isomerase 1, mitochondrial
• UniProt Protein Name: Enoyl-CoA delta isomerase 1, mitochondrial
• Protein Family: Enoyl-CoA delta isomerase
• UniProt Gene Name: ECI1
• UniProt Synonym Gene Names: DCI; D3,D2-enoyl-CoA isomerase
• UniProt Entry Name: ECI1_HUMAN

NCBI Description

This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010]

Uniprot Description

DCI: Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Belongs to the enoyl-CoA hydratase/isomerase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Lipid Metabolism - fatty acid; EC 5.3.3.8; Isomerase

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane

Molecular Function: identical protein binding; dodecenoyl-CoA delta-isomerase activity; intramolecular oxidoreductase activity

Biological Process: fatty acid beta-oxidation; cellular lipid metabolic process