Mouse anti-Human, Mouse RBBP5 Monoclonal Antibody | anti-RBBP5 antibody

RBBP5 Antibody

Cat. Num. AAA9215517

• Gene Names: RBBP5; RBQ3; SWD1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunocytochemistry
• Synonyms: RBBP5; Monoclonal Antibody; RBBP5 Antibody; Purified Mouse Monoclonal Antibody (Mab); RBBP 5; RBBP-5; RBBP5_HUMAN; RBQ 3; RBQ3; Retinoblastoma binding protein 5; Retinoblastoma binding protein RBQ3; Retinoblastoma binding protein5; Retinoblastoma-binding protein 5; Retinoblastoma-binding protein RBQ-3; SWD1; Set1c WD40 repeat protein; homolog.; anti-RBBP5 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Form/Format: PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
• Sequence Length: 500

• Applicable Applications for anti-RBBP5 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: WB ~~ 1:1000; ICC ~~ 1:100
• Antigen Source: Human
• Antigen Type: Recombinant Protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot detection of RBBP5 in C2C12,THP-1,PC12 and 3T3 cell lysates using RBBP5 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.)

Immunocytochemistry (ICC)

(Immunofluorescent analysis of Hela cells fixed with 4% Paraformaldehyde and using anti-RBBP5 mouse mAb (dilution 1:100). DAPI was used to stain nucleus(blue).)

Related Product Information for anti-RBBP5 antibody

In embryonic stem (ES) cells, plays a crucial role in the differentiation potential, particularly along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci, including that mediated by retinoic acid (By similarity). As part of the MLL1/MLL complex, involved in mono-, di- and trimethylation at 'Lys-4' of histone H3. Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation.

Product Categories/Family for anti-RBBP5 antibody

Cancer

References

Saijo M.,et al.Genomics 27:511-519(1995). Ota T.,et al.Nat. Genet. 36:40-45(2004). Gregory S.G.,et al.Nature 441:315-321(2006). Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. Hughes C.M.,et al.Mol. Cell 13:587-597(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 300796323
• NCBI GeneID: 5929
• NCBI Accession #: NP_001180201.1
• NCBI GenBank Nucleotide #: NM_001193272.1
• UniProt Accession #: Q15291; Q7Z6D8; Q8NDZ7; A8K272
• Molecular Weight: 59153
• NCBI Official Full Name: retinoblastoma-binding protein 5 isoform 2
• NCBI Official Synonym Full Names: retinoblastoma binding protein 5
• NCBI Official Symbol: RBBP5
• NCBI Official Synonym Symbols: RBQ3; SWD1
• NCBI Protein Information: retinoblastoma-binding protein 5
• UniProt Protein Name: Retinoblastoma-binding protein 5
• Protein Family: Retinoblastoma-binding protein
• UniProt Gene Name: RBBP5
• UniProt Synonym Gene Names: RBQ3; RBBP-5
• UniProt Entry Name: RBBP5_HUMAN

NCBI Description

This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. The encoded protein binds directly to retinoblastoma protein, which regulates cell proliferation. It interacts preferentially with the underphosphorylated retinoblastoma protein via the E1A-binding pocket B. Three alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

RBBP5: As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Component of the SET1 complex, at least composed of the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30. Core component of several methyltransferase-containing complexes including MLL1/MLL, ASCOM, MLL2/MLL3 and MLL3/MLL4. Each complex is at least composed of ASH2L, RBBP5, DPY30, WDR5, one or more specific histone methyltransferases (MLL, MLL2, MLL3 and MLL4), and the facultative components C16orf53/PA1, C17orf49, CHD8, E2F6, HCFC1, HCFC2, HSP70, INO80C, KDM6A, KANSL1, LAS1L, MAX, MCRS1, MEN1, MGA, MYST1/MOF, NCOA6, PAXIP1/PTIP, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with WDR5 and ASH2L; the interaction is direct. Interacts with WDR82 and SETD1A. Ubiquitously expressed. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: nucleoplasm; histone methyltransferase complex; nucleolus; nucleus

Molecular Function: protein binding; histone lysine N-methyltransferase activity (H3-K4 specific); methylated histone residue binding

Biological Process: establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; transcription, DNA-dependent; response to estrogen stimulus; histone H3-K4 methylation; response to DNA damage stimulus