Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cell using TPD52L1 antibody. Blue: DAPI for nuclear staining.)
Rabbit anti-Human TPD52L1 Polyclonal Antibody | anti-TPD52L1 antibody
TPD52L1 Polyclonal Antibody
Reactivity
Human
Applications
Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
TPD52L1; Polyclonal Antibody; TPD52L1 Polyclonal Antibody; Tumor protein D53; hD53; Tumor protein D52-like 1; anti-TPD52L1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
TPD52L1 polyclonal antibody detects endogenous levels of TPD52L1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
204
Applicable Applications for anti-TPD52L1 antibody
Immunofluorescence (IF)
Application Notes
IF: 1:20-1:50
Immunogen
Recombinant full length Human TPD52L1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cell using TPD52L1 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cell using TPD52L1 antibody. Blue: DAPI for nuclear staining.)
Related Product Information for anti-TPD52L1 antibody
The cell commits to dividing during the G2/M phase transition, and the timing of mitotic entry is tightly regulated to guarantee correct chromosome segregation. D53, also referred to as tumor protein D52-like 1 (TPD52L1), is a coiled-coil motif-bearing member of the D52 tumor protein family. D53 intereacts with 14-3-3, a negative regulator of the G2/M transition, in breast cancer cells. Expression of D53 is highly upregulated at the G2/M phase transition in breast cancer cell lines, and deregulated expression of this protein can adversely affect the completion of mitosis. D53 may function as a signaling intermediate and/or regulator of vesicle trafficking and cell proliferation.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
~ 22kDa
NCBI Official Full Name
Tumor protein D53
UniProt Protein Name
Tumor protein D53
Protein Family
UniProt Gene Name
TPD52L1
UniProt Synonym Gene Names
hD53
UniProt Entry Name
TPD53_HUMAN
Uniprot Description
TPD52L1: a member of the tumor protein D52 (TPD52) family. Contains a coiled-coil domain and may form homo- or hetero-dimer with TPD52 family members. The protein is reported to be involved in cell proliferation and calcium signaling. Interacts with the apoptosis signal-regulating kinase 1 (ASK1) and positively regulates ASK1-induced apoptosis. Multiple alternatively spliced transcript variants have been observed.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 6q22-q23
Cellular Component: perinuclear region of cytoplasm; cytoplasm
Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protein heterodimerization activity
Biological Process: positive regulation of MAP kinase activity; positive regulation of JNK cascade; G2/M transition of mitotic cell cycle; regulation of caspase activity
Similar Products
Product Notes
The TPD52L1 tpd52l1 (Catalog #AAA3011562) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TPD52L1 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TPD52L1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF). IF: 1:20-1:50. Researchers should empirically determine the suitability of the TPD52L1 tpd52l1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TPD52L1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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