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Western Blot (WB) (ARL8A Antibody (Center) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the ARL8A antibody detected the ARL8A protein (arrow).)

Rabbit ARL8A Polyclonal Antibody | anti-ARL8A antibody

ARL8A Antibody (Center)

Gene Names
ARL8A; GIE2; ARL10B
Reactivity
Human, mouse (Predicted Reactivity: Chicken)
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ARL8A; Polyclonal Antibody; ARL8A Antibody (Center); ADP-ribosylation factor-like protein 8A; ADP-ribosylation factor-like protein 10B; Novel small G protein indispensable for equal chromosome segregation 2; ARL10B; GIE2; anti-ARL8A antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Chicken)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ARL8A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-142 amino acids from the Central region of human ARL8A.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
116-142
Sequence Length
186
Applicable Applications for anti-ARL8A antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(ARL8A Antibody (Center) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the ARL8A antibody detected the ARL8A protein (arrow).)

Western Blot (WB) (ARL8A Antibody (Center) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the ARL8A antibody detected the ARL8A protein (arrow).)

Western Blot (WB)

(ARL8A Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the ARL8A antibody detected the ARL8A protein (arrow).)

Western Blot (WB) (ARL8A Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the ARL8A antibody detected the ARL8A protein (arrow).)

Immunohistochemistry (IHC)

(ARL8A Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ARL8A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (ARL8A Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ARL8A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ARL8A Antibody (Center) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (ARL8A Antibody (Center) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-ARL8A antibody
References
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Hofmann, I., et al. J. Cell. Sci. 119 (PT 8), 1494-1503 (2006) :
Okai, T., et al. J. Cell. Sci. 117 (PT 20), 4705-4715 (2004) :
Secombe, J., et al. J. Biol. Chem. 279(17):17126-17133(2004)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21416
NCBI Official Full Name
ADP-ribosylation factor-like protein 8A isoform 2
NCBI Official Synonym Full Names
ADP-ribosylation factor-like 8A
NCBI Official Symbol
ARL8A
NCBI Official Synonym Symbols
GIE2; ARL10B
NCBI Protein Information
ADP-ribosylation factor-like protein 8A
UniProt Protein Name
ADP-ribosylation factor-like protein 8A
UniProt Gene Name
ARL8A
UniProt Synonym Gene Names
ARL10B; GIE2
UniProt Entry Name
ARL8A_HUMAN

Uniprot Description

ARL8A: May play a role in lysosomes motility. Alternatively, may play a role in chromosome segregation. Belongs to the small GTPase superfamily. Arf family.

Protein type: G protein, monomeric; G protein, monomeric, ARF

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: membrane; late endosome membrane; lysosomal membrane; cytoplasm; spindle midzone; midbody

Molecular Function: GTPase activity; GTP binding; beta-tubulin binding; alpha-tubulin binding

Biological Process: mitosis; metabolic process; cell division; small GTPase mediated signal transduction; chromosome segregation

Research Articles on ARL8A

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Product Notes

The ARL8A arl8a (Catalog #AAA9215276) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 116-142. The ARL8A Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Chicken) and may cross-react with other species as described in the data sheet. AAA Biotech's ARL8A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the ARL8A arl8a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARL8A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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