Typical Testing Data/Standard Curve (for reference only)
Rat TAFI ELISA Kit | TAFI elisa kit
Rat TAFI (Thrombin Activatable Fibrinolysis Inhibitor) ELISA Kit
Gene Names
Cpb2; CPR; Cpu; TAFI; AI255929; 1110032P04Rik; 4930405E17Rik
Reactivity
Rat
Synonyms
TAFI; Rat TAFI (Thrombin Activatable Fibrinolysis Inhibitor) ELISA Kit; TAFI elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat TAFI. No significant cross-reactivity or interference between Rat TAFI and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rat TAFI and all the analogues, therefore, cross reaction may still exist.
Sequence Length
422
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
1.563-100ng/mL
Sensitivity
Min: 0.938ng/mL; Max: 100ng/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TAFI elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TAFI in Rat serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TAFI. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat TAFI and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TAFI, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat TAFI. You can calculate the concentration of Rat TAFI in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TAFI. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat TAFI and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TAFI, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat TAFI. You can calculate the concentration of Rat TAFI in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
48,871 Da
NCBI Official Full Name
thrombin-activatable fibrinolysis inhibitor
NCBI Official Synonym Full Names
carboxypeptidase B2 (plasma)
NCBI Official Symbol
Cpb2
NCBI Official Synonym Symbols
CPR; Cpu; TAFI; AI255929; 1110032P04Rik; 4930405E17Rik
NCBI Protein Information
carboxypeptidase B2; carboxypeptidase R; carboxypeptidase U; thrombin-activable fibrinolysis inhibitor; thrombin-activatable fibrinolysis inhibitor
UniProt Protein Name
Carboxypeptidase B2
UniProt Gene Name
Cpb2
UniProt Synonym Gene Names
Tafi; CPR; CPU; TAFI
UniProt Entry Name
CBPB2_MOUSE
Uniprot Description
CPB2: Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down- regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Protease; EC 3.4.17.20; Secreted; Secreted, signal peptide
Cellular Component: extracellular space; extracellular region
Molecular Function: peptidase activity; metallopeptidase activity; carboxypeptidase activity; hydrolase activity; zinc ion binding; metal ion binding; metallocarboxypeptidase activity
Biological Process: fibrinolysis; negative regulation of fibrinolysis; hemostasis; response to heat; blood coagulation; proteolysis
Research Articles on TAFI
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Product Notes
The Rat TAFI cpb2 (Catalog #AAA2511089) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2511089 ELISA Kit recognizes Rat TAFI. It is sometimes possible for the material contained within the vial of "TAFI, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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