Rabbit MeCP2 Polyclonal Antibody | anti-MECP2 antibody

MeCP2 Antibody (N-term)

Cat. Num. AAA9214688

• Gene Names: MECP2; RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13
• Reactivity: Human (Predicted Reactivity: Monkey)
• Applications: Western Blot, ELISA, Flow Cytometry, Functional Assay
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: MeCP2; Polyclonal Antibody; MeCP2 Antibody (N-term); Methyl-CpG-binding protein 2; MeCp-2 protein; MeCp2; MECP2; anti-MECP2 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Monkey)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This MeCP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MeCP2.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 1-30
• Sequence Length: 498

• Applicable Applications for anti-MECP2 antibody: Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of MeCP2 (arrow) using rabbit polyclonal MeCP2 Antibody (E11). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MeCP2 gene.)

Flow Cytometry (FC/FACS)

(MeCP2 Antibody (N-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-MECP2 antibody

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.

Product Categories/Family for anti-MECP2 antibody

Neuroscience

References

dos Santos, J.M., et al., Neurosci. Lett. 379(1):13-16 (2005).
Ylisaukko-Oja, T., et al., Am J Med Genet A 132(2):121-124 (2005).
Schanen, C., et al., Am J Med Genet A 126(2):129-140 (2004).
Shibayama, A., et al., Am. J. Med. Genet. B Neuropsychiatr. Genet. 128(1):50-53 (2004).
Fang, J.Y., et al., World J. Gastroenterol. 10(23):3394-3398 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 160707950
• NCBI GeneID: 4204
• NCBI Accession #: NP_001104262.1; NP_004983.1
• NCBI GenBank Nucleotide #: NM_001110792.1
• UniProt Accession #: P51608; O15233; Q6QHH9; Q7Z384
• Molecular Weight: 52441
• NCBI Official Full Name: methyl-CpG-binding protein 2 isoform 2
• NCBI Official Synonym Full Names: methyl CpG binding protein 2
• NCBI Official Symbol: MECP2
• NCBI Official Synonym Symbols: RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13
• NCBI Protein Information: methyl-CpG-binding protein 2
• UniProt Protein Name: Methyl-CpG-binding protein 2
• Protein Family: Methyl-CpG-binding protein
• UniProt Gene Name: MECP2
• UniProt Synonym Gene Names: MeCp-2 protein; MeCp2
• UniProt Entry Name: MECP2_HUMAN

NCBI Description

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. [provided by RefSeq, Jul 2009]

Uniprot Description

MECP2: Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Interacts with FNBP3. Interacts with CDKL5. Present in all adult somatic tissues tested. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: nucleoplasm; heterochromatin; extracellular space; cytosol; nucleus

Molecular Function: mRNA binding; protein domain specific binding; protein binding; siRNA binding; DNA binding; methyl-CpG binding; double-stranded methylated DNA binding; protein N-terminus binding; chromatin binding; transcription factor binding; transcription corepressor activity; transcription factor activity

Biological Process: catecholamine secretion; startle response; genetic imprinting; behavioral fear response; adult locomotory behavior; positive regulation of transcription, DNA-dependent; chromatin silencing; neurological control of breathing; pathogenesis; negative regulation of transcription from RNA polymerase II promoter; proprioception; sensory perception of pain; neuron maturation; negative regulation of histone methylation; phosphatidylcholine metabolic process; post-embryonic development; negative regulation of histone acetylation; protein localization; synaptogenesis; positive regulation of cell proliferation; dendrite development; cerebellum development; visual learning; negative regulation of neuron apoptosis; histone acetylation; mitochondrial electron transport, ubiquinol to cytochrome c; transcription, DNA-dependent; ventricular system development; glutamine metabolic process; social behavior; respiratory gaseous exchange; cardiolipin metabolic process; histone methylation; positive regulation of synaptogenesis; glucocorticoid metabolic process; regulation of systemic arterial blood pressure by neurological process; long-term memory; inositol metabolic process; response to hypoxia; negative regulation of transcription, DNA-dependent; regulation of excitatory postsynaptic membrane potential

Disease: Rett Syndrome; Mental Retardation, X-linked, Syndromic 13; Autism, Susceptibility To, X-linked 3; Lubs X-linked Mental Retardation Syndrome; Angelman Syndrome; Encephalopathy, Neonatal Severe, Due To Mecp2 Mutations